Manipulation of organic acid biosynthesis and secretion
AssigneesAgriculture Victoria Services PTY LTD, AgResearch Limited
InventorsGerman Spangenberg, Michael Emmerling, Eng Kok Ong, Ramiro Mendez, Stephen Panter, Marcel Labandera
Life of this patent
Estimating the term…
Abstract
The present invention relates to nucleic acid fragments encoding amino acid sequences for organic acid biosynthetic enzymes in plants, and the use thereof for the modification of, for example, organic acid biosynthesis and secretion in plants. In particularly preferred embodiments, the invention relates to the combinatorial expression of citrate synthase (CS) and/or malate dehydrogenase (MDH) and/or phosphoenolpyruvate carboxylase (PEPC) in plants to modify, for example, organic acid synthesis and secretion.
Claims29
1-29. (canceled)
A substantially purified or isolated nucleic acid or nucleic acid fragment encoding an organic acid biosynthesis enzyme polypeptide selected from the group consisting of a citrate synthase (CS) polypeptide;
- a CS-like polypeptide;
- a malate dehydrogenase (MDH) polypeptide;
- a MDH-like polypeptide;
- a phosphoenolpyruvate carboxylase (PEPC) polypeptide;
- and a PEPC-like polypeptide;
- or a functionally active fragment or variant of such a polypeptide, from a clover (Trifolium), medic (Medicago), ryegrass (Lolium) or fescue (Festuca) species.
A nucleic acid or nucleic acid fragment encoding a CS or CS-like polypeptide and including a nucleotide sequence selected from the gloupconsisting of (a) sequences shown in (SEQ ID NOS 1, 3 to 10, 11, 13 to 16, 17, 19, 327, 329 to 335, 336, 338 to 344, 349, 351, and 353;
- (b) complements of the sequences recited in (a);
- (c) sequences antisense to the sequences recited in (a) and (b);
- (d) functionally active fragments and variants of the sequences recited in (a), (b), and (c);
- and (e) RNA sequences corresponding to the sequences recited in (a), (b), (c) and (d).
A nucleic acid or nucleic acid fragment encoding a MDH or MDH-like polypeptide and including a nucleotide sequence selected from the group consisting of (a) sequence shown in SEQ ID NOS 21, 23 to 29;
- 30, 32 to33, 34, 36, 38, 40, 42to 43, 44, 46, 48 to 110, 111, 113, 115, 117 to 182, 183, 185, 205, 207 to 217, 218, 220 to 251, 252, 254 to 270, 271, 273 to 275, 276, 278 to 287, 288, 290 to 292, 293, 295 to 296, 297, 299 to 301, 304 to 305, 306, and 308;
- (b) complements of the sequences recited in (a);
- (c) sequences antisense to the sequences recited in (a) and (b);
- (d) functionally active fragments and variants of the sequences recited in (a), (b) and (c);
- and (e) RNA sequences corresponding to the sequences recited in (a), (b), (c) and (d).
A nucleic acidornucleic acidfi-agment encoding a PEPC or PEPC-like polypeptide and including a nucleotide sequence selected from the group consisting of (a) sequences shown in SEQ ID NOS 187, 189, 191 to 197, 199, 201, 903, 310, 312 to 314, 315, 317 to 318, 319, 321 to 399, 393, 325 and 347;
- (b) complements of the sequences recited in (a);
- (c) sequences antisense to the sequences recited in (a) and (b);
- (d) functionally active fragments and variants of the sequences recited in (a), (b) and (c);
- and (e) RNA sequences corresponding to the sequences recited in (a), (b), (c) and (d).
A substantially purified or isolated polypeptic from a clover (Trifolium), medic (Medicago), ryegrass (Lolium) or fescue (Festuca) species, selected from the group consisting of CS and CS-like, MDH and MDH-like and PEPC and PEPC-like;
- and functionally active fragments and variants thereof.
Dependencies are inferred from the claim text.