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US 2007/0169211 A1Published application
Non-glycosylated human alpha-fetoprotein, methods of production, and uses thereof
AssigneeMerrimack Pharmaceuticals, Inc.
InventorsRobert Mulroy, Ian Krane
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Abstract
The invention features non-glycosylated human alpha-fetoprotein, methods of production, and uses thereof.
Claims23
A nucleic acid molecule encoding non-glycosylated human alpha-fetoprotein (ng.HuAFP), or a nonglycosylated fragment thereof.
A nucleic acid molecule comprising:
- (i) a nucleic acid sequence encoding ng.HuAFP, (ii) a promoter that is operably linked to said ng.HuAFP-encoding sequence that enables expression of ng.HuAFP, and (iii) a leader sequence encoding a protein secretory signal that enables secretion of said ng.HuAFP by a cell.
A method of producing ng.HuAFP, said method comprising the steps of:
- (a) providing a cell transduced with a transgene that comprises:
- (i) a nucleic acid molecule encoding n.g.HuAFP comprising nucleotides 45 through 1874 of the nucleic acid sequence set forth in SEQ ID NO:
- 5, (ii) a promoter that is operably linked to said n.g.HuAFP-encoding molecule and that enables expression of said n.g.HuAFP by said cell, and (iii) a leader sequence encoding a protein secretory signal that enables secretion of said n.g.HuAFP by said cell;
- and
- (b) growing said transduced cell, wherein said cell expresses and secretes said ng.HuAFP.
2 more elements
Dependencies are inferred from the claim text.