Process for the purification of antibodies

A process for the purification of one or more antibodies from a liquid, comprising contacting said liquid with a first chromatography resin including a support to which multi-modal ligands have been immobilised to adsorb the antibodies to the resin, wherein each multi-modal ligand includes at least one cation-exchanging group and at least one aromatic or heteroaromatic ring system;

• adding an eluent to release the antibodies from the resin;
• and contacting the eluate so obtained with a second chromatography resin.

A method for capture of one or more antibodies from a liquid, comprising contacting said liquid with a chromatography resin to adsorb the antibodies to the ligands, which resin is multimodal and comprises a support to which ligands have been immobilised, wherein the resin includes cation-exchanging groups and aromatic or heteroaromatic ring systems present on the same or different ligands, in which systems the ring-forming atoms are selected from the group consisting of carbon (C), sulphur (S) and oxygen (O) atoms.

A process for the purification of one or more antibodies from a liquid, comprising contacting said liquid with a first chromatography resin to adsorb the antibodies to the ligands, which resin is multimodal and comprises a support to which ligands have been immobilised, wherein the resin includes cation-exchanging groups and aromatic or heteroaromatic ring systems present on the same or different ligands;

• adding an eluent to release the antibodies from the resin;
• and contacting the eluate so obtained with a second chromatography resin.

A kit for the purification of one or more antibodies in a liquid, comprising in separate compartments a first chromatography column packed with a chromatography resin, which resin is multimodal and includes a support to which ligands have been immobilised, wherein cation-exchanging groups and aromatic or heteroaromatic ring systems are present on the same or different ligands;

• a second chromatography column packed with a chromatography resin;
• one or more buffers for adsorption and or elution of antibodies;
• and written instructions that teaches the purification of antibodies in a two-step process.

A disposable chromatography column for the purification of antibodies, comprising a multi-modal chromatography resin comprising cation-exchanging groups and aromatic and/or heteroaromatic ring systems on the same or different ligands.

A disposable chromatography column for the purification of antibodies, comprising a multi-modal chromatography resin, wherein each ligand comprises at least one cation-exchanging group and at least one aromatic or heteroaromatic ring system.