Affinity resin
AssigneeReverse Proteomics Research Institute Co., Ltd.
InventorsAkito Tanaka, Takaaki Shiyama, Akira Yamazaki
Life of this patent
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Abstract
A resin which is prepared by polymerizing a monomer component incorporating a hydrophilic spacer, and a ligand-immobilized solid phase carrier obtained by immobilizing a ligand to the resin, are capable of reducing the non-specific adsorption of substances, other than the target molecule for the ligand, which mingle in the sample, to the resin and/or the ligand. Therefore, target molecule search, identification and the like with less noise are enabled.
Claims47
A resin obtained by polymerizing a starting material monomer, wherein the monomer incorporates a hydrophilic spacer, and wherein a ligand is optionally immobilized on the resin.
A compound represented by the formula shown below:
- wherein Ya is a hydrogen atom or an amino-group-protecting group.
A compound represented by the formula shown below:
- wherein Yb is a hydrogen atom or an amino-group-protecting group.
A compound represented by the formula shown below:
- wherein Yb is a hydrogen atom or an amino-group-protecting group.
(canceled)
(canceled)
A screening method for a target molecule, which interacts specifically with a ligand, which method comprises:
- (i) immobilizing a ligand to a resin obtained by polymerizing a starting material monomer, wherein the monomer incorporates a hydrophilic spacer,
- (ii) contacting the ligand-immobilized resin obtained in (i), with a sample, which may or may not comprise the target molecule,
- (iii) identifying and/or analyzing a molecule, which interacts specifically or does not interact specifically with the ligand, and
- (iv) judging the molecule that interacts specifically with the ligand to be a target molecule.
A method of measuring a target molecule, which interacts specifically with a ligand in a sample, which method comprises:
- (i) immobilizing a ligand to a resin obtained by polymerizing a starting material monomer, wherein the monomer incorporates a hydrophilic spacer,
- (ii) contacting the ligand-immobilized resin obtained in (i) with a sample,
- (iii) measuring and/or analyzing a molecule, which interacts specifically or does not interact specifically with the ligand, and
- (iv) measuring a target molecule, which interacts specifically with the ligand.
Dependencies are inferred from the claim text.