Hydrogels for modulating cell migration and matrix deposition
OwnerChad Kennedy, Gholam Ehteshami +2inventors
InventorsChad Kennedy, Gholam Ehteshami, Doris Hom, Stephen Massia
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Abstract
A biodegradable matrix for inducing cell migration therein, wherein two peptides are covalently linked to the matrix, a first peptide being cleavable by natural proteases and the other comprising a cell-attracting peptide. The first peptide is cleavable by tissue metalloproteinases. The second peptide contains the cell-attracting peptide RGD. Also disclosed is an implant containing the biodegradable matrix.
Claims36
A biodegradable matrix for inducing cell migration therein, wherein two peptides are covalently linked to the matrix, a first peptide being cleavable by natural proteases and the other comprising a cell-attracting peptide.
A method of preparing a dextran hydrogel suitable for cellular in-growth, the method comprising
- a. providing a dextran;
- b. combining the dextran with a cleavable peptide and a peptide capable of attracting cells to produce a conjugated dextran;
- c. combining the conjugated dextran with acryloylated dextran;
- d. adding to the dextran mixture a polymerization initiator;
- and
- e. activating the initiator to form a hydro gel.
A method of preparing a hydrogel suitable for promoting cellular in-growth, the method comprising
- a. providing dextran;
- b. combining the dextran with dimethylsulfoxide (DMSO), dimethylaminopyridine (DMAP) and glycol methacrylate (GMA) to form glycidyl methacrylate dextran;
- c. combining the glycidyl methacrylate dextran with acryloylated dextran;
- d. combining the dextran mixture with a polymerization initiator and with at least two peptides, a first peptide capable of attracting cells and a second peptide being degradable by cellular proteases, in a dilute electrolyte solution;
- and
- e. applying energy to polymerize the mixture, thus producing a hydrogel.
The method of 16, wherein step b is followed by mixing at room temperature until the solution is completely dissolved.
The method of 16, wherein the mixing step is followed by adding hydrochloric acid to neutralize the solution and stop the reaction.
The method of 16, wherein step c is followed by dialyzing the acryloylated dextran.
An implant comprising a dextran hydrogel, wherein two peptides are covalently linked to the dextran, a first peptide being cleavable by natural proteases and the other comprising a cell-attracting peptide.
A dextran matrix for inducing cell migration therein, wherein a peptide is covalently linked to the matrix, the peptide being cleavable by natural proteases.
A dextran matrix for inducing cell migration therein, wherein a peptide is covalently linked to the matrix, the peptide comprising a cell-attracting peptide.
Dependencies are inferred from the claim text.