Peptide/Lipid Complex Formation by Co-Lyphilization
OwnerJean-Louis Dasseuxinventor
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Abstract
The invention relates to the formation of peptide/lipid vesicles and complexes through the co-lyophilization of peptides, preferably that are able to adopt an amphipathic alphahelical conformation, and one or more lipids. A single solution which solubilizes both the peptides and lipids or two separate solutions may be lyophilized.
Claims37
A method of preparing a lyophilized peptide/lipid product which comprises co-lyophilizing one or more peptides, which are able to adopt an amphipathic conformation, or peptide analogues, and one or more lipids in a solvent system to form a peptide/lipid product, wherein said product can be rehydrated to form peptide/lipid complexes.
A method of preparing a lyophilized peptide/lipid product which comprises (a) solubilizing at least one amphipathic peptide or peptide analogue in a first solution, (b) solubilizing at least one lipid in a second solution, wherein said second solution is miscible with said first solution, (c) combining said first solution with said second solution to form a peptide/lipid solution, and (d) lyophilizing said peptide/lipid solution so that lyophilized peptide/lipid product is formed which can be rehydrated to form peptide/lipid complexes.
A peptide/lipid complexes formed by the process comprising lyophilizing one or more amphipathic peptides or peptide analogues and at least one lipid in a solvent system to form a dehydrated peptide/lipid product which can be rehydrated to form peptide/lipid complexes.
A sterile, lyophilized composition comprising a sterile preparation of a complex formed between a peptide which can adopt a amphipathic alpha helical conformation, or a peptide analogue and a lipid.
A lyophilized composition which comprises a peptide/lipid complex wherein the peptide is a lipid binding protein or an ApoA1, ApoA-II, ApoA-IV, ApoC-I, ApoC-II, ApoC-III, ApoE or another apoprotein analogue.
A lyophilized composition which comprises a peptide/lipid complex wherein said peptide can adopt an amphipathic alpha helical conformation.
A method of preparing a lyophilized peptide/lipid roduct which comprises:
- co-lyophilizing one or more peptides or peptides analogues, which are able to adopt an amphipathic conformation, with one or more lipids in a ratio of peptide to lipid of from about 2 to about 200 in a solvent system for an amount of time sufficient to form a peptide/lipid product which can be rehydrated to form peptide/lipid complexes in solution.
Dependencies are inferred from the claim text.