Multiplex assays using magnetic and non-magnetic particles
AssigneePerkinElmer LAS, Inc.
InventorsMark Bobrow, Karl Adler, Mack Schermer
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Abstract
Disclosed herein are methods and compositions for multiplexed assays using magnetic and non-magnetic particles. Also disclosed are methods and compositions for performing multi-step assays within a single assay vessel.
Claims34
A method for evaluating multiple samples, the method comprising:
- providing a mixture comprising particles, at least some of which are magnetic particles comprising capture moiety and others are non-magnetic particles comprising capture moiety;
- contacting a sample to at least a portion of the mixture;
- and
- evaluating at least some of the magnetic particles and the non-magnetic particles that were contacted to the sample.
A reaction mixture comprising a mixture comprising particles, at least some of which are magnetic particles comprising capture moiety and others are non-magnetic particles comprising capture moiety.
A multi-step assay method comprising:
- (i) providing an assay vessel comprising multiple compartments, the compartments being arranged in sets;
- (ii) providing particles comprising capture moieties in a first set of the compartments;
- (iii) removing the particles from the first set of the compartments and disposing them in a second set of the compartments, wherein the second set of the compartments contain a first reagent;
- (iv) removing the particles from the second set of compartments and disposing them in a third set of compartments, wherein the third set of compartments contain a second reagent.
A multi-compartment assay vessel comprising a grid of compartments, wherein a first column of compartments comprises magnetic particles, wherein a second column comprises a first reagent, and at least a third column comprises a third reagent, and wherein the assay vessel is suitable for transfer of the magnetic particles from the compartments in the first column to compartments in the second, third, and other columns.
A kit for performing a multi-step reaction, the kit comprising:
- a mixture comprising particles, at least some of which are magnetic particles comprising a capture moiety;
- and
- an assay vessel for performing a multi-step reaction.
Dependencies are inferred from the claim text.