Device for culturing and transporting cells
AssigneeReliance Life Sciences Pvt. Ltd.
InventorsDeepa Ghosh, Sudheer Shenoy
Life of this patent
Estimating the term…
Abstract
A disposable device is provided for culturing and/or packaging and transporting ready-to-use viable cells cultured on membranes, gels or micro porous substrata. The device includes a housing base defining an interior for culturing cells. The membrane to be used for culturing the cells is placed inside the housing base and fixed with a ring. The base is closed by a lid, which is designed to protect the underlying cells during transport as well as minimize the volume of media used during culture, as well as transport. Media leakage is prevented by the silicon gasket as well as the snaps provided in the device.
Claims24
A cell culture device comprising:
- a base, wherein the base comprises:
- a planar top member and one or more side walls, each with an upper and lower edge, wherein the one or more side walls adjoin the planar top member at the upper edge;
- a well formed in the top member;
- a groove surrounding the well between the planar portion of the top member and the well;
- and
- one or more notches in the lower edge of the one or more side walls configured to receive a latching mechanism;
- a cover, comprising:
- a planar top member having an upper and a lower surface, and one or more side walls, each with an upper and lower edge, wherein the one or more side walls adjoin the planar top member at the upper edge;
- a well formed in the top member;
- a ridge formed on the lower surface of the planar top member and surrounding the well;
- and
- one or more latching mechanisms on the lower edge of the one or more side walls;
- and
- a ring structure sized to fit snugly in the bottom of the well of the base and of a height to contain sufficient cell culture media to enable culturing of cells in the well of the base;
- wherein the cover is of similar overall shape as the base and fits snugly over the base, such that when the cover is placed on the base,
- the well of the cover fits into the well of the base and extends into the well of the base to rest against the ring when the ring is in the well of the base;
- the side walls of the cover fit alongside and enclose the side walls of the base;
- at least one latching mechanism in the lower edge of a cover side wall is aligned with a notch in the lower edge of a base side wall;
- and
- the ridge on the lower surface of the planar top member of the cover fits into the groove in the base.
A cell culture and transport device comprising a base and a lid wherein the lid fits loosely over the base to allow air exchange and to prevent microbial contamination for cell growth, and wherein the lid is latchable to the base to form an air-tight and water-tight seal for transport of cultured cells in the same device, and wherein the device further comprises a support to hold a cell culture membrane in the base taut when the lid is latched to the base.
Dependencies are inferred from the claim text.