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US 2007/0166817 A1Published application
System and Method for Applying Reduced Pressure to Cell Culture
AssigneeKCI Licensing, Inc.
InventorsRobert P. Wilkes, Amy K. McNulty, Kristine Kieswetter, Teri D. Feely, Marisa Schmidt
Life of this patent
Estimating the term…
Abstract
A method and system for culturing cells, having a substantially airtight enclosure configured to culture cells. The method and system also have a first conduit configured to provide a reduced pressure to the substantially airtight enclosure and a second conduit configured to provide a culture media to the substantially airtight enclosure.
Claims36
A cell culture system comprising:
- a substantially airtight enclosure configured to culture cells;
- a first conduit in fluid communication with the substantially airtight enclosure, wherein the first conduit is configured to provide a reduced pressure to the substantially airtight enclosure;
- and
- a second conduit in fluid communication with the substantially airtight enclosure, wherein the second conduit is configured to provide a culture media to the substantially airtight enclosure.
1 more element
A sealing system for a cell culture system comprising:
- a peripheral sealing member configured to engage a plate well and configured to engage a cell culture insert, such that a first seal is formed between the peripheral sealing member and the plate well and a second seal is formed between the peripheral sealing member and the cell culture insert;
- and
- an insert sealing assembly configured to engage the cell culture insert such that a third seal is formed between the insert sealing assembly and the cell culture insert.
A method of culturing cells, the method comprising:
- providing a substantially airtight enclosure;
- providing a cell matrix within the substantially airtight enclosure;
- providing a reduced pressure to the substantially airtight enclosure;
- and
- providing a culture media to the substantially airtight enclosure.
2 more elements
A system for culturing cells, the system comprising:
- a plate well;
- a cell culture insert;
- a peripheral sealing member configured to engage a plate well and configured to engage the cell culture insert, such that peripheral sealing member forms a first seal with the plate well and forms a second seal with the cell culture insert;
- and
- an insert sealing assembly configured to engage the cell culture insert such that the insert sealing assembly forms a third seal with the cell culture insert.
2 more elements
Dependencies are inferred from the claim text.