Method for manufacturing protein chip substrate using plasma and protein chip substrate manufactured by the method
AssigneeSungkyunkwan Univ. Foundation For Corporate Coll.
InventorsDong-geun Jung, Sang-hak Yeo, Chang-rok Choi
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Abstract
Disclosed herein are a protein chip substrate and a method for manufacturing the protein chip substrate. The method includes deposition of plasma polymerized ethylenediamine (PPEDA) having an amine group on plasma polymerized cyclohexnane (PPCHex) by inductively coupled plasma-chemical vapor deposition (ICP-CVD), thereby preventing non-specific adsorption of proteins on a slide surface.
Claims17
A method for manufacturing protein chip substrate wherein plasma polymerized ethylenediamine (PPEDA) having an amine group is deposited on plasma polymerized cyclohexnane (PPCHex) by inductively coupled plasma-chemical vapor deposition (ICP-CVD).
A method for manufacturing a protein chip substrate, the method comprising the steps of:
- washing a slide glass with trichloroethylene, acetone and methanol, sequentially;
- arranging the slide glass on a substrate supporter inside a plasma reaction chamber;
- controlling an inner pressure of the plasma reaction chamber by a vacuum device;
- feeding cyclohexane as a precursor together with a carrier gas into the plasma reaction chamber by a bubbler;
- controlling an inner temperature of the plasma reaction chamber by a heater under the substrate supporter;
- applying inductively coupled plasma (ICP) power and slide voltage (SB) power to an outer electrode and the substrate supporter by the RF generator, respectively, to generate plasma between the slide glass and the plasma reaction chamber;
- uniformly depositing a plasma polymersized cyclohexane (PPCHex) film on the slide glass while polymerizing cyclohexane as a precursor by plasma;
- and
- depositing a plasma-polymerized ethylenediamine (PPEDA) film using ethylenediamine (EDA) as a precursor on the PPCHex film by inductively coupled plasma-chemical vapor deposition (ICP-CVD).
A protein chip substrate comprising a plasma polymerized cyclohexnane (PPCHex) film deposited on a substrate by inductively coupled plasma-chemical vapor deposition (ICP-CVD), and a plasma polymerized ethylenediamine (PPEDA) film having an amine group deposited on the PPCHex film.
Dependencies are inferred from the claim text.