Methods and compositions for detecting enzymatic activity
AssigneePerkinElmer LAS, Inc.
InventorsMark Bobrow, Karl Adler, Mack Schermer
Life of this patent
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Abstract
This invention relates to methods and compositions useful in detecting enzymatic activity. Also featured are methods for diagnosing enzyme deficiencies such as metabolic disorders.
Claims45
A method for evaluating multiple enzymatic activities in a sample, the method comprising:
- providing a mixture of encoded particles comprising particles having an attached enzymatic substrate, the enzymatic substrate being identifiable by a particle code, wherein the mixture includes particles for a plurality of different enzymatic substrates;
- contacting the mixture of particles with a sample;
- and
- detecting modification of at least one of the enzymatic substrates attached to the particles for at least some of the encoded particles.
A method for detecting hydrolase activity, the method comprising:
- providing a hydrolase substrate bound to a support;
- contacting the support-bound hydrolase substrate with a sample;
- and
- detecting modification of the support-bound hydrolase substrate.
A method for detecting biotinidase activity, the method comprising:
- providing a biotinidase substrate bound to a support;
- contacting the support-bound biotinidase substrate with a sample;
- and
- detecting modification of the support-bound biotinidase substrate.
A kit for detecting biotinidase activity, the kit comprising:
- (i) a support-bound biotinidase substrate;
- and
- (ii) instructions for detecting biotinidase activity.
A diagnostic method for evaluating biotinidase deficiency in a subject, the method comprising:
- providing a biotinidase substrate bound to a support;
- providing a sample from a subject;
- contacting the support-bound biotinidase substrate with the sample;
- and
- detecting whether modification of the support-bound biotinidase substrate has occurred.
A method of detecting an enzymatic activity that catalyzes a reaction, the method comprising:
- contacting an enzymatic substrate with a sample and a mixture comprising particles, wherein at least a portion of the particles preferentially bind to a reaction component;
- and
- detecting the support-bound reaction component.
Dependencies are inferred from the claim text.