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US 2007/0166798 A1Published application
Isolating chondroitin sulfate
AssigneeCargill, Incorporated
InventorsAnil Khare, Stephen Houliston, Timothy Black
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Abstract
A method of isolating chondroitin sulfate product from a feedstock comprising connective tissue.
Claims19
A method of obtaining chondroitin sulfate comprising:
- (a) providing a feedstock comprising connective tissue;
- (b) digesting the feedstock with a protease to form a liquefied digest and undigested matter;
- (c) treating the liquefied digest with a reagent comprising a divalent hydroxide of an alkaline earth metal at a pH greater than about 10, to form a precipitate comprising protein impurities and a treated liquefied digest;
- (d) separating at least a portion of the precipitate from the treated liquefied digest;
- and
- (e) processing the treated liquefied digest using a membrane to form a permeate and a retentate, wherein the retentate comprises chondroitin sulfate.
3 more elements
A system for obtaining chondroitin sulfate comprising:
- (a) a supply apparatus configured to provide feedstock comprising connective tissue and an amount of protease;
- (b) a digestion vessel connected to the supply apparatus, the digestion vessel configured to breakdown the feedstock into a liquefied digest and undigested matter;
- (c) a reactor connected to the digestion vessel, the reactor configured to treat the liquefied digest with a reagent comprising a divalent hydroxide of an alkaline earth metal;
- and
- (d) a filtration apparatus following the reactor, the apparatus comprising a membrane, and wherein the apparatus is configured to allow a permeate through the membrane and hold a retentate comprising chondroitin sulfate.
2 more elements
Dependencies are inferred from the claim text.