Increasing hemoglobin and other heme protein production in bacteria by co-expression of heme transport genes
OwnerJohn Olson, Douglas Hendersoninventors
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Abstract
The present disclosure relates to methods for increasing heme uptake in a Gram-negative bacterium comprising expressing at least one transgenic heme transport gene in the bacterium. The present disclosure also relates to heme protein production cells comprising at least one transgenic heme transport gene and a heme protein gene and plasmids comprising one or more heme transport genes and a promoter operable to promote expression of the genes by iron depletion or by the addition of an inducer molecule. The present disclosure also relates to systems for heme protein production.
Claims35
A method for increasing heme uptake in a Gram-negative bacterium, the method comprising expressing in the bacterium a nucleic acid having a transgenic heme transport gene.
A heme protein production cell comprising a transgenic heme transport gene and a heme protein gene.
A system for heme protein production comprising:
- a plurality of production cells in a growth media supplemented with heme;
- a first nucleic acid capable of being expressed in the production cells, the first nucleic acid encoding a heme protein;
- and
- a second nucleic acid capable of being expressed in the production cells, the second nucleic acid encoding a transgenic heme transport gene;
- wherein the first nucleic acid and second nucleic acid are co-expressed in the production cells.
A plasmid comprising:
- a heme transport gene;
- and
- a promoter operably linked to the heme transport gene.
Dependencies are inferred from the claim text.