Derivatives of magainin and methods of production thereof
AssigneeShanghai Huayi Biotech Lab
InventorsYukun Sun, Dengxi Wu, Zhiyong Zhu
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Abstract
The present invention provides a Magainin derivative peptide and method of production thereof. Also provided is a pharmaceutically composition comprising said Magainin derivative peptide and pharmaceutically acceptable carrier and/or pharmaceutically compatible binding agents. The Magainin derivative peptide of the present invention having amino acid sequence of the general formula shown as below: Gly-Ile-Gly-Lys-Phe-Leu-His-Ser-Ala-Lys-Lys-Phe-Gly-Lys-Ala-Phe-Val-Gly-Glu-Ile-X-Asn-Y-Z-OH in which: X is an amino acid residue selected from the group consisting of Met, Ile and Leu; Y is an amino acid residue selected from the group consisting of Ser, Lys, Ile, Arg and Leu; and Z is Arg.
Claims16
1-6. (canceled)
An isolated DNA sequence comprising a DNA sequence encoding a peptide of Magainin derivative having amino acid sequence of the general formula shown as below:
- Gly-Ile-Gly-Lys-Phe-Leu-His-Ser-Ala-Lys-Lys-Phe-Gly-Lys-Ala-Phe-Val-Gly-Glu-Ile-X-Asn-Y-Z-OH
- in which:
- X is an amino acid selected from the group consisting of Met, Ile and Leu;
- Y is an amino acid selected from the group consisting of Ser, Lys, Ile, Leu and Arg, and;
- Z is Arg.
An isolated DNA sequence comprising a DNA sequence encoding the fusion peptide of at least two tandemly linked peptides of Magainin derivative having amino acid sequence of the general formula shown as below:
- Gly-Ile-Gly-Lys-Phe-Leu-His-Ser-Ala-Lys-Lys-Phe-Gly-Lys-Ala-Phe-Val-Gly-Glu-Ile-X-Asn-Y-Z-OH
- in which:
- X is an amino acid selected from the group consisting of Met, Ile and Leu;
- Y is an amino acid selected from the group consisting of Ser, Lys, Ile, Leu and Arg, and, Z is Arg.
A method for producing the Magainin derivative peptide of claim 1, said method comprising:
- a. constructing an expression vector comprising at least two tandemly linked DNA sequences encoding said Magainin derivative peptide of claim 1 and a promoter sequence, wherein said promoter sequence is capable of driving the expression of said tandemly linked DNA sequences encoding said Magainin derivative peptide;
- b. expressing said expression vector in a host cell to produce a fusion protein comprising at least two tandemly linked Magainin derivative peptides;
- and
- c. cleaving said fusion protein into separate Magainin derivative peptides with a cleavage reagent.
16-19. (canceled)
A method for the treatment of a patient having need of a Magainin derivative comprising:
- administering to said patient a therapeutically effective amount of the peptide of claim 1.
Dependencies are inferred from the claim text.