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AssigneeSudershan Biotech Ltd.
InventorsVeakata Madhusudhan Mule, Padala Mythili, Karanam Gopalakrishna, Yamala Ramana, Donthi Reddy
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The present invention relates to a recombinant calf-Chymosin protein as set forth in SEQ ID No. 1; a recombinant calf-Chymosin gene as set forth in SEQ ID No. 2 encoding the protein comprising amino acid sequence of SEQ ID NO. 1; an E. coli comprising the recombinant chymosin gene of SEQ ID No. 2; an expression vector pET21b comprising recombinant calf-chymosin gene as set forth in SEQ ID No. 2; and lastly a method for producing recombinant calf-chymosin protein as set forth in SEQ ID No. 1 which comprises steps of isolating calf-chymosin gene, cloning the same in bacterial expression vector pET21b, transforming said cloned vector into cells of E. coli, fermenting said E. coli to produce pro-chymosin, converting said pro-chymosin to chymosin and subsequently recovering the recombinant calf-chymosin.
A recombinant calf-Chymosin protein as set forth in SEQ ID No. 1.
A recombinant calf-Chymosin gene as set forth in SEQ ID No. 2.
An E. coli comprising the recombinant chymosin gene of SEQ ID No. 2.
An expression vector pET21b comprising recombinant calf-chymosin gene as set forth in SEQ ID No. 2.
A method for producing recombinant calf-chymosin protein as set forth in SEQ ID No. 1 which comprises steps of isolating calf-chymosin gene, cloning the same in bacterial expression vector pET21b, transforming said cloned vector into cells of E. coli, fermenting said E. coli to produce pro-chymosin, converting said pro-chymosin to chymosin and subsequently recovering the recombinant calf-chymosin.
Dependencies are inferred from the claim text.