Immunoassay and kit for an early and simultaneous detection of biochemical markers in a patient's sample

A method for detection of myocardial infarction in a patient, comprising:

• a) contacting a sample from the patient with a carrier molecule, the carrier molecule comprising an anti-GPBB monoclonal antibody, an anti-cardiac troponin-I monoclonal antibody, and at least one detection agent,
• b) detecting the concentration of GPBB and cardiac troponin-I in the sample wherein the detection agent binds both the GPBB and troponin-I at binding moieties that are not used by the capture agents;
• and
• c) repeating a) and b) with a new sample for a predetermined time period.

A method of determining the time of onset of mycoardial infarction, comprising:

• a) contacting a sample from a human or animal with a carrier molecule comprising at least two capture agents, each capture agent having binding specificity for different antigens, to form a reaction mixture;
• b) adding at least two detection agents to the reaction mixture;
• and
• c) detecting a concentration of each of at least one heart specific early onset antigen and at least one heart specific late onset antigen in the sample;
• and
• d) determining from the concentrations of the at least one heart specific early onset antigen and the at least one heart specific late onset antigen whether a successive infarction has occurred.