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US 2007/0166769 A1Published application
Bi-directionally cloned random cDNA expression vector libraries, compositions and methods of use
OwnerJames Lorens, Jakob Bogenbergerinventors
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Abstract
The present invention provides random cDNA expression vector libraries, comprising expression vectors which comprise random cDNAs positioned in sense and antisense orientation, which are useful for the delivery and expression of a combination of genetic effector types to host cells. Methods for producing these libraries through bi-directional cloning of random cDNAs are also provided. Also provided herein are methods of using these libraries to screen for agents capable of modulating cell phenotype in desirable ways.
Claims13
1-20. (canceled)
A bi-directionally cloned cDNA expression vector library comprising:
- a) a first plurality of expression vectors, each comprising:
- i. a promoter that is active in a mammalian cell;
- and
- ii. a cDNA fragment that is operably linked to said promoter in a sense orientation;
- and
- b) a second plurality of expression vectors, each comprising:
- i. said promoter;
- and
- ii. a cDNA fragment that is operably linked to said promoter in an antisense orientation.
6 more elements
Dependencies are inferred from the claim text.