Compositions and Methods for Nucleic Acid Extraction from Biological Samples
AssigneeSigma-Aldrich Co.
InventorsScott Weber, Derek Douglas, Carol Kreader
Life of this patent
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Abstract
Methods and compositions for extracting nucleic acids from a biological sample are provided. The extraction compositions contain a protease enzyme such as proteinase K at alkaline pH with little or no surfactant present. Extraction can be efficiently performed in 60 minutes or less at room temperature for certain mammalian tissue samples and at elevated temperatures for certain plant tissues.
Claims14
A method for extracting nucleic acids from a tissue sample obtained from a mammal, the method comprising incubating the sample at room temperature in an extraction composition which contains a protease enzyme and a buffer component which buffers the composition to a pH of 7.5 or greater for an incubation time of not more than 30 minutes.
Dependencies are inferred from the claim text.