US 2007/0166748 A1 · HUMAN DIACYLGLYCEROL ACYLTRANSFERASE 2 (DGAT2) FAMILY MEMBERS AND USES THEREFOR

Abstract

The present invention relates to compositions and methods for the diagnosis and treatment of obesity and related metabolic disorders. The invention provides isolated nucleic acids molecules, designated DGAT2 family member nucleic acid molecules, which encode diacylglycerol acyltransferase family members. The invention also provides recombinant expression vectors containing DGAT2 family member nucleic acid molecules, host cells into which the expression vectors have been introduced, and nonhuman transgenic animals in which a DGAT2 family member gene has been introduced or disrupted. The invention still further provides isolated DGAT2 family member proteins, fusion proteins, antigenic peptides and anti-DGAT2 family member antibodies. Methods of use of the provided DGAT2 family member compositions for screening, diagnostic and therapeutic methods in connection, with obesity disorders are also disclosed.

Claims29

Dependent claims

An isolated nucleic acid molecule selected from the group consisting of:

a) a nucleic acid molecule comprising a nucleotide sequence which is at least 85% identical to the nucleotide sequence of SEQ ID NO:7, SEQ ID NO:19, or SEQ ID NO:61;
b) a nucleic acid molecule comprising a fragment of at least 300 nucleotides of the nucleotide sequence of SEQ ID NO:7, SEQ ID NO:19 or SEQ ID NO:61;
c) a nucleic acid molecule which encodes a polypeptide comprising the amino acid sequence selected from the group consisting of SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:12, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:20 and SEQ ID NO:62;
d) a nucleic acid molecule which encodes a fragment of a polypeptide comprising the amino acid sequence of SEQ ID NO:8, SEQ ID NO:20, or SEQ ID NO:62, wherein the fragment comprises at least 15 contiguous amino acids of SEQ ID NO:8, SEQ ID NO:20 or SEQ ID NO:62;
e) a nucleic acid molecule which encodes a naturally occurring allelic variant of a polypeptide comprising the amino acid sequence of SEQ ID NO:8, SEQ ID NO:20 or SEQ ID NO:62, wherein the nucleic acid molecule hybridizes to a nucleic acid molecule comprising a sequence consisting of SEQ ID NO:7, SEQ ID NO:19, or SEQ ID NO:61 or a complement thereof, under stringent conditions;
and
f) a nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:11, SEQ ID NO:13, SEQ ID NO:15, SEQ ID NO:17, SEQ ID NO:19, and SEQ ID NO:61.

4 more elements

A method of determining acyltransferase activity of a polypeptide comprising:

a) combining a sample comprising an acyltransferase polypeptide with a first fatty acyl coA substrate and a second acylglyceride substrate under conditions suitable for enzyme activity and
b) determining the amount of acylglycerol product formation, thereby determining acyltransferase activity,
wherein one substrate is biotinylated and the other substrate is radiolabeled, and wherein the resulting acylglycerol product is detected using biotin capture and radiometric detection.

Dependencies are inferred from the claim text.

Human diacylglycerol acyltransferase 2 (dgat2) family members and uses therefor

Life of this patent

Human diacylglycerol acyltransferase 2 (dgat2) family members and uses therefor

Life of this patent

Abstract

Claims29