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US 2007/0166744 A1Published application
Analytical method and instrument
AssigneeRoche Molecular Systems, Inc.
InventorRolf Knobel
Life of this patent
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Abstract
Subjects of the invention are a method for determination of an analyte using a particular algorithm based on a mathematical model for transforming measurement data into a growth curve and an analytical instrument comprising a computing unit for more precise determination of an analyte.
Claims15
A method for determining the presence of a nucleic acid in a sample, comprising:
- providing a reaction mixture containing said nucleic acid,
- detecting signals from said reaction mixture in known intervals,
- transforming said signals into measurement data, and
- creating from said measurement data a growth curve through a mathematical algorithm,
- wherein said mathematical algorithm comprises a mathematical growth curve model formula depending on between 5 and 11 parameters and wherein the optimal values of said parameters are determined by applying a regression fitting algorithm to said measurement data, said growth curve model formula comprising
- a background curve estimation,
- a saturation curve estimation, and
- an intermediate growth curve estimation according to the formula
- 1/(1+exp(−p4 (x−p5)))/(1+exp(−p6(x−p7)))
- wherein
- p4 is the slope at the inflection point of a first multiplicative sigmoid function representing exponential growth,
- p5 is the inflection point of a first multiplicative sigmoid function,
- p6 is the slope at the inflection point of a second multiplicative sigmoid function representing saturation growth, and
- p7 is the inflection point of a second multiplicative sigmoid function.
11 more elements
An analytical instrument for determining the presence of an analyte, comprising:
- a signal detection unit,
- a signal to measurement data transformation unit,
- a measurement data storage,
- a measurement data to growth curve transformation unit,
- a growth curve to digital data transformation unit, and
- a growth curve comparison unit,
- wherein said measurement data to growth curve transformation unit comprises a computer loaded with a fully automatic algorithm for non-linear regression fitting of a growth curve to the measurement data using a mathematical algorithm comprising a mathematical growth curve model formula depending on between 5 and 11 parameters, wherein the optimal values of said parameters are determined by applying a regression fitting algorithm to said measurement data and wherein said growth curve model formula at least comprises
- a background curve estimation,
- a saturation curve estimation, and
- an intermediate growth curve estimation according to the formula
- 1/(1+exp(−p4(x−p5)))/(1+exp(−p6(x−p7)))
- wherein
- p4 is the slope at the inflection point of a first multiplicative sigmoid function representing exponential growth,
- p5 is the inflection point of a first multiplicative sigmoid function,
- p6 is the slope at the inflection point of a second multiplicative sigmoid function representing saturation growth, and
- p7 is the inflection point of a second multiplicative sigmoid function.
13 more elements
A computer program product comprising a set of computer instructions stored on a computer readable medium for determining the presence of an analyte from measurement data, said set of computer instructions being executable by a processor, comprising:
- creating a growth curve from measurement data through a mathematical algorithm,
- wherein said mathematical algorithm comprises a mathematical growth curve model formula depending on between 5 and 11 parameters and wherein the optimal values of said parameters are determined by applying a regression fitting algorithm to said measurement data, said growth curve model formula comprising
- a background curve estimation,
- a saturation curve estimation, and
- an intermediate growth curve estimation according to the formula
- 1/(1+exp(−p4(x−p5)))/(1+exp(−p6(x−p7)))
- wherein
- p4 is the slope at the inflection point of a first multiplicative sigmoid function representing exponential growth,
- p5 is the inflection point of a first multiplicative sigmoid function,
- p6 is the slope at the inflection point of a second multiplicative sigmoid function representing saturation growth, and
- p7 is the inflection point of a second multiplicative sigmoid function.
8 more elements
Dependencies are inferred from the claim text.