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US 2007/0166725 A1Published application
Multiplexed diagnostic platform for point-of care pathogen detection
AssigneeThe Regents of the University of California
InventorsMary McBride, Benjamin Hindson, Steve Brown
Life of this patent
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Abstract
The invention provides a system for high-throughput multiplex analysis of target samples. A sample and reagent delivery unit is operatively connected to a thermal cycler for amplification of target nucleic acids. Microspheres are hybridized to the resulting amplicons in the thermal cycler. A flow cytometer is operatively connected to the thermal cycler or optionally a bead trap for washing the microspheres.
Claims22
A system comprising:
- a) a reagent chamber;
- b) a sample chamber;
- c) a sample preparation chamber;
- d) a thermalcycler comprising a conductive copper section defining a cavity for receiving a sample, wherein said thermalcycler is operatively connected to said cavity;
- e) a flow cytometer;
- f) at least one channel connecting said reagent chamber to said sample preparation chamber and at least one channel connecting said sample chamber to said sample preparation chamber;
- g) at least one channel from said sample preparation chamber to said thermal cycler;
- and
- h) at least one channel from said thermal cycler to said flow cytometer.
6 more elements
A nucleic acid assay apparatus for analyzing a sample using a reagent, comprising:
- a thermal cycler comprising a copper section defining a cavity for receiving sample, a sample and reagent delivery unit operatively connected to said thermal cycler for delivering the sample and the reagent to said thermal cycler, a bead trap operatively connected to said thermal cycler, and a flow cytometer operatively connected to said bead trap.
Dependencies are inferred from the claim text.