Hybridization-triggered fluorescent detection of nucleic acids
AssigneeEpoch Biosciences, Inc.
InventorsRobert Dempcy, Irina Afonina, Nicolaas Vermeulen
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Abstract
Compositions and methods for fluorescent detection of nucleic acids are provided. The compositions can be detected by fluorescence when hybridized to a nucleic acid containing a target sequence, but are non-fluorescent in the non-hybridized state. Alternatively, the fluorescence properties of the compositions change in a detectable manner upon hybridization to a nucleic acid containing a target sequence. Methods for synthesis and methods of use of the compositions are also provided.
Claims8
1-41. (canceled)
A method for detecting fluorescence in a cyanine dye having a minor groove binder attached thereto, said method comprising:
- providing a cyanine dye having a minor groove binder attached thereto, which is essentially nonfluorescent in the absence of double stranded nucleic acid;
- and
- contacting said cyanine dye with double stranded nucleic acid to trigger hybridization fluorescence.
Dependencies are inferred from the claim text.