Method for evaluating rnai activity and mirna activity
AssigneeBio- Think Tank Co., Ltd.,
InventorsYuki Naito, Kumiko Tel, Shuhel Zenno, Kaoru Saigo
Life of this patent
Estimating the term…
Abstract
The present invention provides methods whereby an RNAi activity and a miRNA activity can be evaluated simply and conveniently. The RNAi activity or the miRNA activity is evaluated by supplying a target-expressing molecule, which is a polynucleotide containing at least a target sequence and an expression regulatory region regulating the expression of an RNA containing the target sequence, and a subject nucleic acid molecule to be evaluated on whether or not it has RNAi activity on the RNA containing the target sequence into an expression system in which the target-expressing molecule can express the RNA containing the target sequence.
Claims13
1) A method for evaluating RNAi activity, comprising the steps of:
- supplying a target-expressing molecule, which is a polynucleotide containing at least a target sequence and an expression regulatory region which regulates an expression of an RNA containing said target sequence, and
- a subject nucleic acid molecule to be evaluated on whether or not the subject nucleic acid molecule has an RNAi activity on said RNA containing the target sequence
- into an expression system in which said target-expressing molecule is capable of expressing said RNA containing the target sequence;
- and
- detecting whether or not said RNA containing said target sequence has been cleaved.
4) The method for evaluating RNAi activity of claim, wherein said target-expressing molecule comprises a sequence encoding an expression product detectable as a reporter at a location whereby said reporter is to be transcribed as an mRNA integrated with the target sequence, the method comprising a step of detecting the expression of said reporter to detect whether or not the RNA has been cleaved.
9) A method for evaluating miRNA activity, comprising the steps of:
- supplying a target-expressing molecule, which is a polynucleotide comprising:
- at least a target sequence, an expression regulatory region regulating an expression of an RNA containing said target sequence, and a sequence encoding an expression product detectable as a reporter at a location whereby said reporter is to be transcribed as an mRNA integrated with said target sequence;
- and
- a subject nucleic acid molecule to be evaluated on whether or not said subject nucleic acid molecule has an RNAi activity on an RNA containing said target sequence
- into an expression system in which said target-expressing molecule is capable of expressing said RNA containing the target sequence;
- and
- detecting whether or not said RNA containing the target sequence has been cleaved.
Dependencies are inferred from the claim text.