Methods for preparing gene chips and use thereof
AssigneeIntegragen
InventorsPierre Lindenbaum, Philippe Gesnouin
Life of this patent
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Abstract
The invention relates to methods and compositions for producing biochips as well as to the use of these biochips in diverse fields, from functional genomics to diagnosis, for example, particularly in research or in the medical field. The invention also relates to tools and methods for selecting polynucleotides that permit the production of improved biochips. The inventive biochips contain, in particular, BAC clones.
Claims21
1-21. (canceled)
A method for producing a biochip including a support on which is immobilized a set of polynucleotides, which method comprises the steps of:
- (i) obtaining BAC clones including a nucleic insert corresponding to or specific to a portion of a human genome,
- (ii) selecting, from the BAC clones thus obtained, a set of BAC clones including a single nucleic insert in the human genome, the BAC clones of the selected set including nucleic inserts substantially uniformly distributed over the human genome, the selection step comprising:
- (a) eliminating non-single clones;
- (b) eliminating clones sharing a same STS;
- (c) eliminating STS which are marked at least two different places on the human genome;
- (d) classifying clones as a function of their position on the genome to defining neighbouring clones;
- and
- (e) eliminating clones by applying an iterative method so as to obtain in particular substantially uniform distribution of the finally selected clones over the human genome;
- and
- (iii) depositing, on a support, the selected clones or the nucleic inserts contained in said clones, or part of them, under conditions enabling the deposited clones or inserts to hybridise with a nucleic acid having a complementary sequence.
The method for producing a biochip including a support on which has been immobilized a set of polynucleotides, which method comprising the steps of:
- (i) obtaining BAC clones including a nucleic insert corresponding to or specific to a portion of a human genome;
- (ii) selecting, from the BAC clones thus obtained, a set of BAC clones including a single insert in the human genome, BAC clones of the selected set including nucleic inserts substantially uniformly distributed over the human genome, the selection step comprising:
- (a) eliminating non-single clones;
- (b) eliminating clones sharing a same STS;
- (c) eliminating STS which are marked at at least two different places on the human genome;
- (d) classifying clones as a function of their position on the genome to defining neighbouring clones;
- and
- (e) eliminating clones by applying an iterative method so as to obtain in particular substantially uniform distribution of the finally selected clones over the human genome;
- (iii) amplifying the BAC clones of the selected set and/or the nucleic inserts that they contain;
- and
- (iv) depositing, on a support, the selected clones or the nucleic inserts contained in said clones, or part of them, under conditions enabling deposited clones or inserts to hybridise with a nucleic acid having a complementary sequence.
A biochip that comprises a support on which is immobilized a set of BAC clones including a nucleic insert corresponding to or specific to a portion of a human genome, each clone including a single insert in the human genome and carrying an STS not shared by any other insert of the BAC clones of the set, the BAC clones of the set including nucleic inserts substantially uniformly distributed over the human genome.
A method for identifying or locating a nucleic acid on the human genome, comprising placing a nucleic acid in contact with a biochip, in conditions enabling hybridisation between complementary sequences, detection of a hybridisation signal, and identification of the position of the nucleic acid on the genome by identification of the clones involved in the hybridisations, wherein the biochip comprises a support on which is immobilized a set of BAC clones including a nucleic insert corresponding to or specific to a portion of a human genome, each clone including a single insert in the human genome and carrying an STS not shared by any other insert of the BAC clones of the set, the BAC clones of the set including nucleic inserts substantially uniformly distributed over the human genome.
A method for identifying genes associated with a given character trait, comprising (i) identifying fragments of nucleic acids identical between at least two samples taken from subjects having a common character trait, and (ii) hybridising fragments thus identified on a biochip and, (iii) detecting a hybridisation signal, making it possible to locate the fragment(s) on the human genome and thus to identify one or more genes present therein, associated with said character trait, wherein the biochip comprises a support on which is immobilized a set of BAC clones including a nucleic insert corresponding to or specific to a portion of a human genome, each clone including a single insert in the human genome and carrying an STS not shared by any other insert of the BAC clones of the set, the BAC clones of the set including nucleic inserts substantially uniformly distributed over the human genome.
Dependencies are inferred from the claim text.