Methods for detecting flavivirus infection
AssigneesThe Govt. of the U.S.A. as represented by the Secretary of the Dept. of Health & Human Services, Centers for Disease Control and Prevention
InventorGwong-Jen Chang
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Abstract
The present invention encompasses isolated nucleic acids containing transcriptional units which encode a signal sequence of one flavivirus and an immunogenic flavivirus antigen of a second flavivirus. The invention further encompasses a nucleic acid and protein vaccine and the use of the vaccine to immunize a subject against flavivirus infection. The invention also provides antigens encoded by nucleic acids of the invention, antibodies elicited in response to the antigens and use of the antigens and/or antibodies in detecting flavivirus or diagnosing flavivirus infection.
Claims19
A method of detecting a flavivirus antibody in a sample, comprising:
- (a) contacting the sample with a flavivirus antigen,
- which flavivirus antigen is an immunogenic flavivirus antigen produced by expressing a transcriptional unit encoding a signal sequence of a structural protein of a first flavivirus and an immunogenic flavivirus antigen of a second flavivirus, wherein the transcriptional unit directs the synthesis of the immunogenic flavivirus antigen,
- under conditions whereby an antigen/antibody complex can form;
- and
- (b) detecting antigen/antibody complex formation, thereby detecting a flavivirus antibody in the sample.
Dependencies are inferred from the claim text.