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US 2007/0166432 A1Published application
Amylase
AssigneeNovozymes A/S
InventorsYe Liu, Tang Lan, Tina Spendler, Mary Stringer
Life of this patent
Estimating the term…
Abstract
The inventors have identified amylases in fungal strains of Valsaria and found that the amylase can increase the shelf life of baked products. Particularly, the novel amylase in com-bination with a maltogenic amylase further improves the softness of bread crumb without hav-ing detrimental effects on elasticity.
Claims20
1-17. (canceled)
An isolated polypeptide which has amylase activity and has an amino acid sequence which comprises:
- a) a catalytic core sequence encoded by a DNA sequence present in a plasmid in E. coli DSM 16113 or in Valsaria rubricosa CBS 848.96,
- b) a sequence as shown in positions 1-439 or position 1-566 of SEQ ID NO 2 or 19;
- c) a sequence which has at least 70% identity to the sequence defined in (a) or (b), or
- d) a sequence encoded by a nucleic acid sequence which hybridizes with the complementary strand of nucleotides 146-1462 of SEQ ID NO:
- 1 or nucleotides 76-1392 of SEQ ID NO:
- 18 under hybridization conditions comprising prehybridizing in a solution of 5×SSC, 5×Denhardt's solution, 0.5% SDS and 100 micrograms/ml of denatured sonicated salmon sperm DNA, followed by hybridization in the same solution for 12 hours at 55° C., and then washing twice for 30 minutes in 2×SSC, 0.5% SDS at a temperature of 60° C.
3 more elements
A process for preparing a dough-based product, comprising adding a first and a second amylase to a dough, leavening, and heating the dough, wherein:
- g) the first amylase retains more than 50% activity after 15 min incubation at 62° C. in 50 mM sodium acetate, 1 mM CaCl2, pH 5.7, and has an amino acid sequence comprising a catalytic module and carbohydrate-binding module, and
- h) the second amylase is an exo-acting amylase.
Dependencies are inferred from the claim text.