Stabilized lyophilized blood platelets
OwnerIrina Bakaltchevainventor
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Abstract
A lyophilized platelet preparation, which is preferably a platelet enhanced plasma preparation, was obtained by stabilizing separated platelets with glyceraldehyde. In contrast to fixed preparations of cells, the platelet preparation is made and provided without toxins. Not fixing the platelets according to the invention provides increases utility and allows for the ability to change volume. The platelets are combined with a glyceraldehyde analog for a few hours at slightly elevated temperatures (about 35° C.) and then freeze dried and on reconstitution with distilled water exhibit morphological and physiological properties similar to that of native platelets, and superior to untreated, lyophilized platelets.
Claims20
A lyophilized preparation of blood platelets wherein, prior to lyophilization, said blood platelets are stabilized with glyceraldehyde, genipin, glyoxal, an analog thereof, or a combination thereof.
A reconstituted lyophilized platelet preparation, wherein subsequent to reconstitution, said platelets exhibit a size distribution and freedom from aggregation that is substantially indistinguishable from control platelets that have not been subject to lyophilization.
A method of preparing lyophilized platelets, comprising stabilizing platelets from a donor in the presence of a glyceraldehyde analog at 30-40° C. for a period of 1-3 hours, followed by washing, suspension in a pH buffered protein matrix, and freeze drying the resuspended platelets.
Dependencies are inferred from the claim text.