Compositions and methods for promoting attachment of cells of endothelial cell lineage to medical devices

A method for coating at least one metallic surface of a medical device, the method comprising contacting the at least one metallic surface of the medical device with a biofunctional coating composition to form a coating on the at least one surface;

• wherein the biofunctional coating composition comprises at least one surface-binding domain and at least one endothelial-binding domain;
• wherein the at least one surface-binding domain comprises a peptide consisting essentially of an amino acid sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, and a combination thereof;
• wherein the at least one endothelial-binding domain comprises a peptide consisting essentially of an amino acid sequence selected from the group consisting of SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:26, SEQ ID NO:27, SEQ ID NO:28, SEQ ID NO:29, SEQ ID NO:30, SEQ ID NO:31, SEQ ID NO:32, SEQ ID NO:33, SEQ ID NO:34, SEQ ID NO:35, SEQ ID NO:36, SEQ ID NO:37, SEQ ID NO:38, SEQ ID NO:39, SEQ ID NO:40, SEQ ID NO:41, SEQ ID NO:42, SEQ ID NO:43, SEQ ID NO:44, SEQ ID NO:45, SEQ ID NO:46, and a combination thereof;
• wherein the at least one surface-binding domain is coupled to the at least one endothelial-binding domain;
• and wherein the at least one endothelial-binding domain is in an amount effective in the coating for adhering cells of endothelial cell lineage to the at least one metallic surface.

A method for promoting endothelialization of at least one metallic surface of a medical device, the method comprising contacting the at least one metallic surface of the medical device with a biofunctional coating composition to form a coating on the at least one metallic surface;

• wherein the biofunctional coating composition comprises at least one surface-binding domain coupled to at least one endothelial-binding domain;
• wherein the at least one surface-binding domain comprises a peptide consisting essentially of an amino acid sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, and a combination thereof;
• wherein the at least one endothelial-binding domain comprises a peptide consisting essentially of an amino acid sequence selected from the group consisting of SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:26, SEQ ID NO:27, SEQ ID NO:28, SEQ ID NO:29, SEQ ID NO:30, SEQ ID NO:31, SEQ ID NO:32, SEQ ID NO:33, SEQ ID NO:34, SEQ ID NO:35, SEQ ID NO:36, SEQ ID NO:37, SEQ ID NO:38, SEQ ID NO:39, SEQ ID NO:40, SEQ ID NO:41, SEQ ID NO:42, SEQ ID NO:43, SEQ ID NO:44, SEQ ID NO:45, SEQ ID NO:46, and a combination thereof;
• wherein the at least one endothelial-binding domain is in an amount effective in the coating for attaching cells of endothelial cell lineage to the at least one surface;
• wherein the at least one surface is contacted with cells of endothelial cell lineage so that cells of endothelial cell lineage attach to the coating, in promoting endothelialization of the at least one surface.

A method for manufacturing a medical device, the method comprising contacting at least one metallic surface of a medical device with a biofunctional coating composition in forming at least one coated surface;

• and contacting the at least one coated surface with cells of endothelial cell lineage;
• wherein the biofunctional coating composition comprises at least one surface-binding domain and at least one endothelial-cell binding domain;
• wherein the at least one surface-binding domain comprises a peptide consisting essentially of an amino acid sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, and a combination thereof;
• wherein the at least one endothelial-binding domain comprises a peptide consisting essentially of an amino acid sequence selected from the group consisting of SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:26, SEQ ID NO:27, SEQ ID NO:28, SEQ ID NO:29, SEQ ID NO:30, SEQ ID NO:31, SEQ ID NO:32, SEQ ID NO:33, SEQ ID NO:34, SEQ ID NO:35, SEQ ID NO:36, SEQ ID NO:37, SEQ ID NO:38, SEQ ID NO:39, SEQ ID NO:40, SEQ ID NO:41, SEQ ID NO:42, SEQ ID NO:43, SEQ ID NO:44, SEQ ID NO:45, SEQ ID NO:46, and a combination thereof;
• and wherein the at least one surface-binding domain and the at least one endothelial-cell binding domain are coupled together.

A medical device comprising at least one metallic surface of the medical device coated with a biofunctional coating composition, wherein the biofunctional coating composition comprises at least one surface-binding domain and at least one endothelial-cell binding domain;

• wherein the at least one surface-binding domain comprises a peptide consisting essentially of an amino acid sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, and a combination thereof;
• wherein the at least one endothelial-binding domain comprises a peptide consisting essentially of an amino acid sequence selected from the group consisting of SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:26, SEQ ID NO:27, SEQ ID NO:28, SEQ ID NO:29, SEQ ID NO:30, SEQ ID NO:31, SEQ ID NO:32, SEQ ID NO:33, SEQ ID NO:34, SEQ ID NO:35, SEQ ID NO:36, SEQ ID NO:37, SEQ ID NO:38, SEQ ID NO:39, SEQ ID NO:40, SEQ ID NO:41, SEQ ID NO:42, SEQ ID NO:43, SEQ ID NO:44, SEQ ID NO:45, SEQ ID NO:46, and a combination thereof;
• and wherein the at least one surface-binding domain and the at least one endothelial-cell binding domain are coupled together.