gM-NEGATIVE EHV-MUTANTS WITHOUT HETEROLOGOUS ELEMENTS
AssigneeBoehringer Ingelheim Vetmedica GmbH
InventorsAntonie Neubauer, Christina Ziegler
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Abstract
The present invention relates to the field of animal health and in particular of Equine Herpes Viruses (EHV) wherein the gene encoding the protein gM is absent, and which is free of heterologous elements. Further aspects of the invention relate to pharmaceutical compositions comprising said viruses, uses thereof, and methods for the prophylaxis and treatment of EHV infections. The invention also relates to pharmaceutical compositions comprising the combination of EHV-1 and EHV-4 viruses wherein the gene encoding the protein gM is absent and which is free of heterologous elements.
Claims31
A recombinant Equine Herpes Virus (EHV) wherein the protein gM is absent, and wherein said EHV is free of heterologous elements.
A method for obtaining a recombinant EHV, comprising the steps of:
- a) isolating a wild-type EHV;
- b) establishing a plasmid encoding the EHV gM gene, optionally with flanking sequences;
- c) generating a complementing cell line expressing gM or parts thereof;
- d) establishing an EH virus carrying a GFP-encoding cassette insert in its gM coding sequence by co-transfecting the complementing cell line of step b) with EHV-nucleic acid and a plasmid encoding gM which is interrupted by a GFP-encoding cassette insert;
- e) deleting the GFP-encoding cassette;
- and
- f) selecting for the EHV clones wherein the GFP-encoding cassette is successfully deleted.
Dependencies are inferred from the claim text.