Method for growing stem cells

A method for growing stem cells comprising the steps of

• providing a stem cell with a supporter cell selected from the group consisting of keratinocytic stem cells, lung and tracheal epithelial cells, bone marrow and hepatic stroma cells, neural-glial precursor cells, tissue cells, and “spore”-like stem cells,
• the supporter cell being genetically modified by a vector comprising (i) a gene for interleukines, protooncogenes, oncogenes, cell cycle control genes, signal transduction genes, and/or cell based growth factors and (ii) a tetracycline regulatable expression system, in order to provide externally regulatable interactions between the supporter cell and the stem cell and
• applying an external signal for starting or stopping the interactions.

A supporter cell, the supporter cell being selected from the group consisting of keratinocytic stem cells, lung and tracheal epithelial cells, bone marrow and hepatic stroma cells, neural-glial precursor cells, tissue cells, and “spore”-like stem cells,

• the supporter cell being genetically modified by a vector comprising (i) a gene for interleukines, protooncogenes, oncogenes, cell cycle control genes, signal transduction genes, and/or cell based growth factors and (ii) a regulatable expression system, in order to provide externally regulatable interactions between the supporter cell and the stem cell and,
• the supporter cell being genetically modified, genetically mutated, and/or modified using molecular and cellular breeding in order to change tet-on to tet-off and vice versa, to change oncogenicity, to change trans-lineage-commitment, and to change trans-species specificity.

A cell line obtainable by transforming a cell with a vector,

• the cell being selected from the group consisting of keratinocytic stem cells, lung and tracheal epithelial cells, bone marrow and hepatic stroma cells, neural-glial precursor cells, tissue cells, and “spore”-like stem cells, and
• the vector comprising (i) a gene for interleukines, protooncogenes, oncogenes, cell cycle control genes, signal transduction genes, and/or cell based growth factors and (ii) a tetracycline regulatable expression system.