Lineage Restricted Glial Precursors from the Central Nervous System
AssigneeUniversity of Utah Research Foundation
InventorsMahendra Rao, Mark Noble, Margot Mayer-Proschel
Life of this patent
Estimating the term…
Abstract
A glial precursor cell population from mammalian central nervous system has been isolated. These A2B5+ E-NCAM− glial-restricted precursor (GRP) cells are capable of differentiating into oligodendrocytes, A2B5+ process-bearing astrocytes, and A2B5− fibroblast-like astrocytes, but not into neurons. GRP cells can be maintained by regeneration in culture. GRP cells differ from oligodendrocyte-type-2 astrocyte (O-2A) progenitor cells in growth factor requirements, morphology, and progeny. Methods of use of GRP cells are also disclosed.
Claims25
An isolated, pure population of mammalian CNS glial restricted precursor cells that is capable of generating oligodendrocytes and at least two distinct populations of astrocytes.
A method for treating a neurological or neurodegenerative disease comprising administering to a mammal in need of such treatment an effective amount of glial restricted precursor cells or derivatives thereof or mixtures thereof.
A method for continuously propagating glial restricted precursor cells comprising the steps of:
- (a) providing said glial restricted precursor cells;
- and
- (b) culturing said glial restricted precursor cells in vitro in the presence of minimal essential salts and effective amounts of platelet derived growth factor and fibroblast growth factor.
Dependencies are inferred from the claim text.