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OwnerBrian Seed, Mason Freeman +4inventors
InventorsBrian Seed, Mason Freeman, Alexander Kovtun, Masahiro Murakawa, Eun-Chung Park, Xinzhong Wang
Estimating the term…
Disclosed are replicatable viral DNA vectors encoding a site-specific DNA-altering enzyme and a DNA target recognized by the enzyme, the enzyme selectively converting, in a cell expressing the enzyme, the DNA vector to a rearranged form. The invention further relates to methods for assembling recombinant adenoviral DNAs. These methods include the steps of: (a) providing a first linearized DNA vector including a restriction site and a cos site and a second linearized DNA vector including the restriction site, an adenoviral nucleic acid molecule, and a cos site; and (b) ligating the first and second linearized DNA vectors, the ligation assembling a recombinant adenoviral DNA.
A method of gene therapy comprising the administration to a patient in need of gene therapy a therapeutically effective amount of a vector which is expressed in said patient, wherein said vector comprises, in the 5′ to 3′ direction,
A method of gene therapy comprising the administration to a patient in need of gene therapy a therapeutically effective amount of a pharmaceutical composition, said composition comprising a biologically suitable vehicle for administration and a vector, said vector comprising, in the 5′ to 3′ direction,
A method of gene therapy comprising the administration to a patient in need of gene therapy a therapeutically effective amount of a population of cells transfected with a vector, said vector comprising, in the 5′ to 3′ direction,
Dependencies are inferred from the claim text.