Disposable reactor module and detection system
AssigneeInstantlabs Medical Diagnostic Corp.
InventorDongqing Li
Life of this patent
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Abstract
A disposable reactor module, monitoring/optical detection system and related hardware for, inter alia, chemical reactions including Polymerase Chain Reactions.
Claims31
A disposable reactor module comprising:
- a substantially non-reflective, thermally conductive substrate;
- and
- a layer of polymer on the substrate with the layer of polymer having at least one reaction well for receiving a fluid sample and with the polymer being chemically inert and non-adherent to DNA.
A method for real-time monitoring of a temperature-controlled chemical reaction involving fluorescence emissions, the method comprising the steps of:
- a) providing at least one fluid sample in a disposable reactor module comprising a substrate and a layer of polymer on the substrate, with the layer of polymer having at least one reaction well for receiving the sample;
- b) sealing at least one reaction well;
- c) heating and cooling the reactor module to allow a chemical reaction to occur;
- d) directing excitation wavelengths to the sample to create fluorescence emissions;
- e) capturing the fluorescence emissions;
- and
- f) processing the fluorescence emissions to monitor the chemical reaction.
A miniature multiplex fluorescence detection system for detecting fluorescence emissions from at least one sample on a reactor module having a plurality of reaction wells, the system comprising:
- at least one light source coupled to the reaction wells, for generating light at excitation wavelengths;
- at least one detector for receiving detection wavelengths from the reaction wells;
- and
- an optical switching device, coupled between the detector and the reaction wells, to direct emissions of fluorescence to the detector.
A device for real-time monitoring of a temperature-controlled chemical reaction involving fluorescence emission-detection, the device comprising:
- a multiplex fluorescence detection system for detecting fluorescence emissions from fluid samples contained in reaction wells, the system comprising:
- at least one light source coupled to the reaction wells, for generating light at excitation wavelengths;
- at least one detector for receiving detection wavelengths from the reaction wells;
- and
- an optical switching device, coupled between the detector and the reaction wells, to direct emissions of fluorescence to the detector;
- a heating and cooling module for modulating a temperature of the samples;
- and
- a control module for controlling the fluorescence detection system and monitoring the chemical reaction by processing the fluorescence emissions.
Dependencies are inferred from the claim text.