Self-sealing high-temperature biochemical reaction apparatus and method for the same

A self-sealing high-temperature biochemical reaction apparatus, comprising:

• a first substrate having a first surface formed with a microfluidic channel area, said microfluific channel area including a main channel chamber, an inlet sub-channel and an outlet sub-channel, wherein said main channel chamber has an inlet and an outlet respectively communicated with said inlet sub-channel and outlet sub-channel, and inner diameters of said inlet sub-channel and outlet sub-channel are smaller than that of said main channel chamber;
• a heater disposed over a second surface of said first substrate corresponding to said main channel chamber;
• and
• a second substrate disposed over said first surface of said first substrate such that said microfluidic channel area becomes a closed channel area, and said second substrate has an inlet and an outlet respectively communicated with said inlet sub-channel and outlet sub-channel;
• wherein during a biochemical reaction of a reaction reagent filled in said micofluidic channel area, said reaction reagent itself seal said inlet sub-channel and outlet sub-channel.

A self-sealing method for a high-temperature biochemical reaction, comprising:

• providing a microfluidic chip having a main channel chamber, an inlet sub-channel and an outlet sub-channel, said microfluific channel area having a main channel chamber, an inlet sub-channel and an outlet sub-channel, wherein said main channel chamber has an inlet and an outlet respectively communicated with said inlet sub-channel and outlet sub-channel, and inner diameters of said inlet sub-channel and outlet sub-channel are smaller than that of said main channel chamber;
• introducing reaction reagent fluid into said inlet sub-channel, main channel chamber and said outlet sub-channel;
• detecting a temperature of said main channel chamber and accordingly generating a feedback signal;
• and
• outputting a control signal depending on the feedback signal to control said main channel chamber to reach a reaction temperature such that the reaction reagent fluid itself seals said inlet sub-channel and outlet sub-channel.