Purification of immunoglobulins
OwnerGrunnar Glad, Bo-Lennart Johansson +2inventors
InventorsGrunnar Glad, Bo-Lennart Johansson, Jean-Luc Maloisel, Nils Norrman
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Abstract
The present invention relates to a separation matrix comprised of a porous support to which ligands have been immobilised, wherein said ligands comprise at least one aliphatic sulphonamide. The nitrogen of the sulphonamide may be a secondary or tertiary amine. The invention also relates to a chromatography column that contains the described separation matrix, as well as to a method of isolating immunoglobulin-like compounds by adsorption to a separation matrix that comprises aliphatic sulphonamide ligands.
Claims28
A separation matrix comprising
- (a) a porous support;
- and
- (b) ligands including one or more sulphonamides wherein an R group of the sulphonyl is an aliphatic compound;
- wherein said ligands are immobilized, optionally via spacer arms, on said porous support.
A process of preparing a matrix for separation of antibodies, which method comprises a first step of immobilising amines and/or polyamines to a porous support and a subsequent step of sulphonylating said amines to provide aliphatic sulphonamide ligands.
A process of preparing a matrix for separation of antibodies, which method comprises a first step of activating a porous support and a subsequent step of attaching sulphonamides to the activated sites via their sulphurs to provide aliphatic sulphonamide ligands.
A method of isolating antibodies from a liquid, which method comprises the steps of
- (a) providing a liquid that comprises at least one antibody;
- (b) contacting said liquid with a separation matrix, which comprises one or more aliphatic sulphonamide ligands, to adsorb one or more antibodies to said matrix;
- and, optionally,
- (c) passing an eluent over said matrix to release one or more antibodies;
- and
- (d) recovering at least one antibody from a fraction of the eluent.
Dependencies are inferred from the claim text.