Sperm Cell Processing and Preservation Systems
AssigneeXy, LLC
InventorsWilliam Maxwell, Fiona Hollinshead, Justine O'Brien, Gareth Evans
Life of this patent
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Abstract
Semen and sperm cell processing and preservation systems, and methods of producing a mammal and methods of producing mammalian embryos are disclosed. The present invention is directed to sperm cell preservation, fertilization, and insemination, maintaining or enhancing sperm quality and addressing one or more sperm cell characteristics, such as viability, motility, functionality, fertilization rates, and pregnancy rates. Further, sperm cell characteristics may be addressed within the context of various collection, handling, separation, storage, transportation, usage, fertilization, or insemination techniques.
Claims53
A method of producing a mammal comprising the steps of:
- obtaining sperm cells from a male of a nonhuman mammalian species;
- cryopreserving said obtained sperm cells;
- thawing said cryopreserved sperm cells;
- processing said thawed sperm cells;
- cryopreserving said processed sperm cells to yield twice-cryopreserved processed sperm cells, wherein an increased percentage of said twice-cryopreserved processed sperm cells, when thawed, are motile, compared to said thawed sperm cells;
- thawing said twice-cryopreserved processed sperm cells to yield thawed twice-cryopreserved processed sperm cells;
- fertilizing at least one egg from a female of said non-human mammalian species with said thawed twice-cryopreserved processed sperm cells;
- producing a mammal from said at least one fertilized egg.
A method of producing a mammal, comprising the steps of:
- obtaining sperm cells;
- cryopreserving said sperm cells;
- thawing said sperm cells;
- cryopreserving said sperm cells;
- fertilizing at least one egg with said sperm cells;
- and
- producing a mammal from said at least one fertilized egg.
A method of producing a mammalian embryo, comprising the steps of:
- obtaining sperm cells;
- cryopreserving said sperm cells;
- thawing said sperm cells;
- cryopreserving said sperm cells;
- fertilizing at least one egg with said sperm cells;
- and
- producing a mammalian embryo from said at least one fertilized egg.
A method of producing a mammal, comprising the steps of:
- obtaining sperm cells;
- cryopreserving said sperm cells;
- thawing said sperm cells;
- cryopreserving said sperm cells;
- fertilizing in vitro said at least one egg with said sperm cells;
- transferring said at least one fertilized egg to a recipient female;
- and
- producing a mammal from said at least one fertilized egg.
A method of producing a mammal, comprising the steps of:
- obtaining sperm cells;
- cryopreserving said sperm cells;
- thawing said sperm cells;
- cryopreserving said sperm cells;
- inseminating at least one female of a species of said mammal with said sperm cells;
- fertilizing at least one egg with said sperm cells;
- and
- producing a mammal from said at least one fertilized egg.
Dependencies are inferred from the claim text.