Equine protozoal myeloencephalitis vaccine
AssigneeWyeth
InventorsRocky Bigbie, Terry Ng, Joseph Whalen
Life of this patent
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Abstract
The present invention provides an immunogenically active component comprising inactivated Sarcocystis neurona cells and/or inactivated Neospora hughesi cells; antigens derived therefrom; DNA derived therefrom; or a mixture; or in combination with other vaccine components thereof. Further provided are vaccine compositions useful for preventing or ameliorating equine protozoal myeloencephalitis infection and disease and a method for the cell culture propagation of protozoan parasites.
Claims25
An immunogenically active component for preventing or ameliorating equine protozoal myoencephalitis infection or disease which comprises:
- tachyzoite antibody-inducing inactivated Neospora hughesi cells,
- a mixture of merozoite antibody-inducing inactivated Sarcocystis neurona cells and tachyzoite antibody-inducing inactivated Neospora hughesi cells,
- a merozoite antibody-inducing antigen derived from Sarcocystis neurona cells,
- a tachyzoite antibody-inducing antigen derived from Neospora hughesi cells,
- DNA derived from Sarcocystis neurona cells,
- DNA derived from Neospora hughesi cells,
- or a mixture thereof.
(canceled)
A vaccine composition for the prevention or amelioration of equine protozoal myoencephalitis disease in equines comprising:
- a first immunogenically active component comprising merozoite antibody-inducing inactivated Sarcocystis neurona cells, a merozoite antibody-inducing antigen derived from said cells, DNA derived from said cells capable of inducing a merozoite antibody immune response or a mixture thereof,
- a second immunogenically active component comprising tachyzoite antibody-inducing inactivated Neospora hughesi cells, a tachyzoite antibody-inducing antigen derived from said cells, DNA derived from said cells capable of inducing a tachyzoite antibody immune response, or a mixture thereof,
- a pharmacologically acceptable carrier and optionally an immunogenically stimulating adjuvant.
A method for the cell culture propagation of Sarcocystis neurona or Neospora hughesi protozoan parasite which comprises:
- a) growing a monolayer of cells having a confluency of 80%-100%;
- b) refeeding said cells with supplemented growth media;
- c) inoculating said cells with merozoites or tachyzoites;
- d) holding the inoculated cells for 4-12 days;
- e) decanting the supplemented growth media from the inoculated cells;
- and
- f) refeeding said cells a second time with supplemented growth media.
Dependencies are inferred from the claim text.