High throughput screening method for antimicrobial formulations
OwnerJohn Novak, James Yuaninventors
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Abstract
Methods and systems are provided for increasing the speed and throughput at which the antimicrobial effects of chemical formulations can be evaluated. Generally, the invention provides a method for screening antimicrobial chemical formulations, comprising depositing microorganisms on a slide, depositing chemical formulations onto the deposited microorganisms, removing the chemical formulations from the deposited microorganisms after a given treatment time, depositing fluorescent dyes onto the deposited microorganisms after removing the chemical formulations, and scanning the slide for fluorescence to evaluate viability of the microorganisms after depositing the fluorescent dyes.
Claims35
A method for screening antimicrobial chemical formulations, comprising:
- a) depositing microorganisms on a slide;
- b) depositing chemical formulations onto the deposited microorganisms;
- c) removing the chemical formulations from the deposited microorganisms after a given treatment time;
- d) depositing fluorescent dyes onto the deposited microorganisms after removing the chemical formulations;
- and
- e) scanning the slide for fluorescence to evaluate viability of the microorganisms after depositing the fluorescent dyes.
A method for screening antimicrobial chemical formulations, comprising:
- a) placing a slide in an atmosphere controlled chamber;
- b) depositing microorganisms on a slide;
- c) depositing chemical formulations onto the deposited microorganisms;
- d) removing the chemical formulations from the deposited microorganisms after a given treatment time;
- e) depositing fluorescent dyes onto the deposited microorganisms after removing the chemical formulations;
- and
- f) scanning the slide for fluorescence to evaluate viability of the microorganisms after depositing the fluorescent dyes.
A system for evaluating the antimicrobial effects of numerous chemical formulations, comprising:
- a) a microarray printer to deposit a plurality of spots containing combinations of chemical formulations, microorganisms, and fluorescent dyes onto a slide;
- b) a microarray scanner to detect live and dead microorganisms in each spot through fluorescence markers;
- and
- c) an execution unit which, upon executing code, is configured to analyze the fluorescence marker data of each spot collected from the microarray scanner.
Dependencies are inferred from the claim text.