Novel gluconate dehydratase
AssigneeMitsui Chemicals, Inc.
InventorsHitoki Miyake, Toshifumi Yamaki, Toshihiro Oikawa, Takeshi Nakamura, Hiroki Ishibashi, Yasushi Fukuiri, Atsushi Sakuma, Hironori Komatsu, Tomoyuki Ando, Kazuhiko Togashi, Hideki Umetani
Life of this patent
Estimating the term…
Abstract
A novel gluconate dehydratase derived from Achromobacter xylosoxidans and a gene encoding the gluconate dehydratase are provided. By reacting the gluconate dehydratase or a transformed cell containing the gene with an aldonic acid, the corresponding 2-keto-3-deoxyaldonic acid can be efficiently produced.
Claims13
1-10. (canceled)
A process for converting an aldonic acid into a corresponding 2-keto-3-deoxyaldonic acid, the process comprising the step of converting the aldonic acid into the 2-keto-3-deoxyaldonic acid in a water-based medium with one selected from the group consisting of a gluconate dehydratase capable of dehydrating D-gluconic acid to produce 2-keto-3-deoxy-D-gluconic acid, and processed products from the gluconate dehydratase:
- said gluconate dehydratase maintaining at least 95% of the enzyme activity thereof after being allowed to stand in 30 mM tris(hydroxymethyl) aminomethane buffer solution with a pH of about 8.5 containing 1 mM of sodium D-gluconate and 1 mM of magnesium chloride at 55° C. for 2 hours.
A process for producing a 2-deoxyaldonic acid, comprising the steps of:
- reacting an aldonic acid or a salt of the aldonic acid with one selected from the group consisting of a gluconate dehydratase capable of dehydrating D-gluconic acid to produce 2-keto-3-deoxy-D-gluconic acid, and processed products from the gluconate dehydratase, in a water-based medium to convert the aldonic acid or the salt into a 2-keto-3-deoxyaldonic acid;
- and
- reacting the 2-keto-3-deoxyaldonic acid with an oxidizing agent in a water-based medium to decarboxylate and to reduce the carbon number by 1, thereby producing the 2-deoxyaldonic acid:
- said gluconate dehydratase maintaining at least 95% of the enzyme activity thereof after being allowed to stand in 30 mM tris(hydroxymethyl) aminomethane buffer solution with a pH of about 8.5 containing 1 mM of sodium D-gluconate and 1 mM of magnesium chloride at 55° C. for 2 hours.
A process for producing a 2-deoxyaldose, comprising the steps of:
- reacting an aldonic acid or a salt of the aldonic acid with one selected from the group consisting of a gluconate dehydratase capable of dehydrating D-gluconic acid to produce 2-keto-3-deoxy-D-gluconic acid, and processed products from the gluconate dehydratase, in a water-based medium to convert the aldonic acid or the salt into a 2-keto-3-deoxyaldonic acid;
- reacting the 2-keto-3-deoxyaldonic acid with a reducing agent in a water-based medium to prepare a 2-hydroxy-3-deoxyaldonic acid;
- and
- reacting the 2-hydroxy-3-deoxyaldonic acid with an oxidizing agent in a water-based medium to decarboxylate and to reduce the carbon number by 1, thereby producing the 2-deoxyaldose
- said gluconate dehydratase maintaining at least 95% of the enzyme activity thereof after being allowed to stand in 30 mM tris(hydroxymethyl) aminomethane buffer solution with a pH of about 8.5 containing 1 mM of sodium D-gluconate and 1 mM of magnesium chloride at 55° C. for 2 hours.
A process for converting an aldonic acid into a corresponding 2-keto-3-deoxyaldonic acid, the process comprising the step of converting the aldonic acid into the 2-keto-3-deoxyaldonic acid in a water-based medium with one selected from the group consisting of a transformed cell prepared by transforming a host cell with a plasmid containing a gene encoding a gluconate dehydratase capable of dehydrating D-gluconic acid to produce 2-keto-3-deoxy-D-gluconic acid, and process products from the transformed cell:
- said gluconate dehydratase maintaining at least 95% of the enzyme activity thereof after being allowed to stand in 30 mM tris(hydroxymethyl) aminomethane buffer solution with a pH of about 8.5 containing 1 mM of sodium D-gluconate and 1 mM of magnesium chloride at 55° C. for 2 hours.
A process for producing a 2-deoxyaldonic acid, comprising the steps of:
- reacting an aldonic acid or a salt of the aldonic acid with one selected from the group consisting of a transformed cell prepared by transforming a host cell with a plasmid containing a gene encoding a gluconate dehydratase capable of dehydrating D-gluconic acid to produce 2-keto-3-deoxy-D-gluconic acid, and processed products from the transformed cell, in a water-based medium to convert the aldonic acid or the salt into a 2-keto-3-deoxyaldonic acid;
- and
- reacting the 2-keto-3-deoxyaldonic acid with an oxidizing agent in a water-based medium to decarboxylate and to reduce the carbon number by 1, thereby producing the 2-deoxyaldonic acid:
- said gluconate dehydratase maintaining at least 95% of the enzyme activity thereof after being allowed to stand in 30 mM tris(hydroxymethyl) aminomethane buffer solution with a pH of about 8.5 containing 1 mM of sodium D-gluconate and 1 mM of magnesium chloride at 55° C. for 2 hours.
A process for producing a 2-deoxyaldose, comprising the steps of:
- reacting an aldonic acid or a salt of the aldonic acid with one selected from the group consisting of the transformed cell prepared by transforming a host cell with a plasmid containing a gene encoding a gluconate dehydratase capable of dehydrating D-gluconic acid to produce 2-keto-3-deoxy-D-gluconic acid, and processed products from the transformed cell, in a water-based medium to convert the aldonic acid or the salt into a 2-keto-3-deoxyaldonic acid;
- reacting the 2-keto-3-deoxyaldonic acid with a reducing agent in a water-based medium to prepare a 2-hydroxy-3-deoxyaldonic acid;
- and
- reacting the 2-hydroxy-3-deoxyaldonic acid with an oxidizing agent in a water-based medium to decarboxylate and to reduce the carbon number by 1, thereby producing the 2-deoxyaldose:
- said gluconate dehydratase maintaining at least 95% of the enzyme activity thereof after being allowed to stand in 30 mM tris(hydroxymethyl) aminomethane buffer solution with a pH of about 8.5 containing 1 mM of sodium D-gluconate and 1 mM of magnesium chloride at 55° C. for 2 hours.
Dependencies are inferred from the claim text.