Nucleic acid delivery
AssigneesCanji, Inc., Innovata Limited
InventorsSusan Conroy, Engler Heidrun, Daniel Maneval
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Abstract
The present invention provides formulations and methods to enhance the delivery of nucleic acids to cells. Formulations comprising dextrin polymers in combination with sugars provide enhanced delivery of nucleic acids, particularly eucaryotic expression vectors, demonstrate enhanced delivery of nucleic acids to cells in vivo. The present invention also provides methods of treatment in combination with such formulations.
Claims36
A method to deliver nucleic acid to a cell wherein the nucleic acid is carried in a solution comprising dextrin and at least one sugar, the osmolarity of which corresponds substantially to the physiological osmolarity of the milieu surrounding the cell.
The method according to claim 19 wherein the divalent cation concentration is between 0.2-3.0 mM.
A composition for delivery of a recombinant viral vector to a cell comprising a recombinant viral vector in a solution comprising dextrin and at least one sugar, the osmolarity of which corresponds substantially to the physiological osmolarity of the milieu surrounding the cell.
A pharmaceutical formulation comprising a recombinant adenoviral vector, a dextrin, a sugar and a divalent cation.
A method to deliver nucleic acid to a cell comprising:
- providing a solution of dextrin, a sugar, a divalent cation and nucleic acid and contacting the solution with a cell to be transfected or transformed.
Dependencies are inferred from the claim text.