In ovo vaccine against infectious bursal disease
AssigneeUniversity of Maryland Biotechnology Institute
InventorVikram Vakharia
Life of this patent
Estimating the term…
Abstract
The present invention relates to a non pathogenic vaccine comprising a recombinant Infectious Bursal Disease virus that includes a recombinant Segment A, designated as rD78GLSNSΔ, that includes sequences from D78 and GLS strains and wherein the NS protein is not expressed.
Claims5
A recombinant virus, deficient in the expression of VP5 nonstructural protein (NS) and characterized by growing one log lower than the parental viruses, exhibits decreased cytotoxic and apoptotic effects in cell culture.
A non-pathogenic vaccine comprising a recombinant IBDV comprising a Segment A designated as rD78GLSNSΔ (SEQ ID NO:
- 5) that includes nucleotides sequences from D78 and GLS strains and wherein the NS protein is not expressed.
An in ovo method of vaccination of poultry in the presence of maternal immunity against IBDV, the method comprising:
- introducing in ovo a vaccine comprising a recombinant virus comprising at least Segment A (SEQ ID NO:
- 5) modified to include nucleotide sequences from D78 and GLS strains and wherein the NS protein is not expressed and the vaccine is delivered in an amount sufficient to protective against STC-IBDV challenge.
A live, non-pathogenic recombinant IBD virus and method of producing same for use as a vaccine to protect against IBDV, the method comprising the following steps:
- (a) preparing cDNA of infectious bursal disease virus genome segments A and B, wherein the segment A (SEQ ID NO:
- 5) is modified to prevent expression of NS protein, wherein the cDNA contains epitopic determinants from D78 and/or GLS strains;
- (b) transcribing said cDNA to produce synthetic RNA transcripts,
- (c) transfecting host cells with said synthetic RNA transcripts,
- (d) incubating said host cells in a culture medium, and
- (e) isolating live, nonpathogenic, infectious bursal disease virus from said culture medium.
Dependencies are inferred from the claim text.