AMP Assay
OwnerKristian Janssoninventor
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Abstract
The invention provides a method for detecting AMP, which method comprises treating a sample with adenylate kinase, nucleoside-diphosphate kinase, and a phosphate donor under conditions such that when AMP is present in the sample ATP is formed; and detecting the ATP so formed, preferably with the light-producing luciferase-luciferin reaction.
Claims11
A method for detecting AMP, characterized in that it comprises (a) treating a sample with adenylate kinase, nucleoside-diphosphate kinase, and a phosphate donor under conditions such that when AMP is present in the sample, ATP is formed;
- and (b) detetecting the ATP formed.
A reagent for detecting AMP, characterized in that it comprises adenylate kinase, nucleoside-diphosphate kinase, and a phosphate donor.
A kit for detecting AMP, characterized in that it comprises in a packaged combination adenylate kinase, nucleoside-diphosphate kinase, and a phosphate donor.
Dependencies are inferred from the claim text.