High throughput one-hybrid system

A method of identifying a protein that binds to a bait element, the method comprising:

• obtaining a cell whose genome comprises one or more integrated bait-reporter constructs, wherein each of the one or more bait-reporter constructs comprises
• (a) a single copy of a bait element flanked by lambda recombination sites, wherein the bait element comprises at least 250 base pairs;
• and
• (b) a reporter gene;
• transforming the cell with an expression vector encoding a fusion protein comprising an activation domain;
• and
• assessing activation of the reporter gene,
• wherein activation of the reporter gene indicates that the activation domain fusion protein has bound to the bait element.