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US 2006/0275877 A1Published application
Method of producing a low molecular weight organic compound in a cell
AssigneeBLMaps
InventorsJoergen Hansen, Thomas Andersen, Finn Okkels
Life of this patent
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Abstract
A method of producing a low molecular weight organic compound (e.g. a plant or bacteria secondary metabolite) in increased yields involving use of a microorganism cell, which comprises a gene involved in the biosynthesis pathway leading to a low molecular weight organic aglycon compound and a glycosyltransferase gene capable of glycosylating the produced aglycon.
Claims20
A method of producing a low molecular weight organic aglycon compound comprising following steps:
- a) fermenting a microorganism cell in a suitable medium where the microorganism is capable of growing, which comprises a gene encoding a product involved in the biosynthesis pathway leading to a low molecular weight organic aglycon compound and a glycosyltransferase gene encoding a glycosyltransferase capable of glycosylating the produced aglycon, under suitable conditions wherein the cell produces the aglycon and the corresponding glycosylated form of the aglycon;
- b) deglycosylating the glycosylated form of the aglycon;
- and
- c) recovering the aglycon compound;
- (i) wherein the low molecular weight organic aglycon compound has a molecular weight from 50 to 3000, and
- (ii) wherein the glycosyltransferase is a glycosyltransferase capable of conjugating a sugar to the aglycon compound.
3 more elements
A low molecular weight organic aglycon compound made from the process comprising:
- a) fermenting a microorganism cell in a suitable medium where the microorganism is capable of growing, which comprises a gene encoding a product involved in the biosynthesis pathway leading to a low molecular weight organic aglycon compound and a glycosyltransferase gene encoding a glycosyltransferase capable of glycosylating the produced aglycon, under suitable conditions wherein the cell produces the aglycon and the corresponding glycosylated form of the aglycon;
- b) deglycosylating the glycosylated form of the aglycon;
- and
- c) recovering the aglycon compound;
- (i) wherein the low molecular weight organic aglycon compound has a molecular weight from 50 to 3000, and
- (ii) wherein the glycosyltransferase is a glycosyltransferase capable of conjugating a sugar to the aglycon compound.
3 more elements
Dependencies are inferred from the claim text.