Recombinant double-stranded RNA phage, and use of the same
AssigneeUniversity of Maryland Biotechnology Institute
InventorDavid Hone
Life of this patent
Estimating the term…
Abstract
A recombinant double stranded RNA (dsRNA) phage expresses dsRNA-encoded genes in eukaryote cells. Recombinant dsRNA phage are useful for the expression of dsRNA expression cassettes encoding passenger genes, such as, but not restricted to, vaccine antigens, bioactive proteins, immunoregulatory proteins, antisense RNAs, and catalytic RNAs in eukaryotic cells or tissues. Methods are provided to deliver recombinant dsRNA phage to eukaryotic cells and tissues, either by direct administration, formulated in lipid or polylactide-coglycolide, or by utilizing a bacterial vaccine vector.
Claims19
1-21. (canceled)
A live bacterium comprising a double stranded RNA (dsRNA) phage that is transcribed and expresses at least one genetic sequence in eukaryote cells, wherein the dsRNA phage includes a cap independent translation enhancer (CITE), and at least one genetic sequence that is expressed in a eukaryote cell, wherein said CITE and said at least one genetic sequence are functionally linked and are incorporated into one or more dsRNA segments in the dsRNA.
A method for immunizing a subject comprising infecting said subject with a live bacterium comprising a double stranded RNA (dsRNA) phage that is transcribed and expresses at least one genetic sequence that yields at least one immunogen, wherein the dsRNA phage includes a cap independent translation enhancer (CITE), and at least one genetic sequence that expresses said immunogen in a eukaryote cell, wherein said CITE and said at least one genetic sequence are functionally linked and are incorporated into one or more dsRNA segments in the dsRNA.
A method of vaccinating an animal, comprising the steps of:
- pulsing dendritic cells with a double stranded RNA (dsRNA) phage that expresses at least one genetic sequence in eukaryote cells, comprising a cap independent translation enhancer (CITE), and at least one genetic sequence that is expressed in a eukaryote cell, wherein said CITE and said at least one genetic sequence are functionally linked and are incorporated into one or more dsRNA segments in the dsRNA;
- and
- injecting said dentritic cells into an animal.
A method of vaccinating an animal, comprising the step of providing the animal with a live attenuated bacterium harboring a double stranded RNA (dsRNA) phage that expresses at least one genetic sequence in eukaryote cells, comprising a cap independent translation enhancer (CITE), and at least one genetic sequence that is expressed in a eukaryote cell, wherein said CITE and said at least one genetic sequence are functionally linked and are incorporated into one or more dsRNA segments in the dsRNA.
An anthrax vaccine, comprising:
- a live bacterium harboring a double stranded RNA (dsRNA) phage that expresses at least one genetic sequence that expresses Bacillus anthrax lethal factor in eukaryote cells, said dsRNA includes in combination a cap independent translation enhancer (CITE), and at least one genetic sequence that expresses Bacillus anthrax lethal factor, wherein said CITE and said at least one genetic sequence are functionally linked and are incorporated into one or more dsRNA segments in the dsRNA.
A tuberculosis vaccine, comprising:
- a live bacterium harboring a double stranded RNA (dsRNA) phage that expresses at least one genetic sequence that expresses a tuberculosis antigen in eukaryote cells, said dsRNA includes in combination a cap independent translation enhancer (CITE), and at least one genetic sequence that expresses a tuberculosis antigen, wherein said CITE and said at least one genetic sequence are functionally linked and are incorporated into one or more dsRNA segments in the dsRNA.
Dependencies are inferred from the claim text.