Methods of monitoring functional status of transplants using gene panels
AssigneeExpression Diagnostics, Inc.
InventorsSteven Rosenberg, Preeti Lal, Kirk Fry, Tod Klingler, Dirk Walther, Robert Woodward
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Abstract
Methods useful in monitoring the functional status of a transplant in a patient by detecting the expression levels of gene panels are described herein. Algorithms for analysis of the expression for monitoring the functional status of transplants are also described.
Claims20
A method of diagnosing or monitoring the functional status of a transplant in a subject comprising:
- a) detecting the expression levels of all genes of a diagnostic gene set in the subject wherein the diagnostic gene set comprises at least one gene from each of at least two gene tables selected from the group consisting of table 1, table 2, table 3, table 4, table 5, table 6, table 7, table 8, table 9, table 10, table 11;
- table 12, table 13, table 14, table 15 and table 16;
- and
- b) diagnosing or monitoring the functional status of the transplant in the subject based upon the expression levels of the genes in the diagnostic gene set.
A method of generating a probe set for diagnosing or monitoring transplant rejection in a subject comprising:
- a) generating a diagnostic gene set by selecting at least one gene from each of at least two gene tables selected from the group consisting of table 1, table 2, table 3, table 4, table 5, table 6, table 7, table 8, table 9, table 10, table 11;
- table 12, table 13, table 14, table 15 and table 16;
- and
- b) generating a probe set by creating at least one probe that specifically detects the expression level for each gene in the diagnostic gene set.
A probe set comprising at least one probe for detection of expression of at least one gene from each of at least two gene tables selected from the group consisting of table 1, table 2, table 3, table 4, table 5, table 6, table 7, table 8, table 9, table 10, table 11;
- table 12, table 13, table 14, table 15 and table 16.
A method of diagnosing or monitoring the functional status of a transplant in a subject comprising:
- a) detecting the expression levels of all genes of a diagnostic gene set in a patient wherein the diagnostic gene set comprises at least one gene from each of at least two gene clusters selected from the group consisting of Cell-Surface Mediated Signaling Cluster, Inflammation Cluster, Steroid Responsive Gene Cluster, Early Activation Cluster, Heart Failure Cluster, Hematopoiesis Cluster, Megakaryocytes Cluster, T/B Cell Regulation Cluster, Transcription Control Cluster, T Cell Cluster, Inflammatory Cell Recruitment Cluster, Transcription Factor Related Cluster, Dendritic Cell Maturation Cluster, Cell Activation Cluster, Cytotoxic T Cell Cluster, and Bone Marrow Stromal Cell Migration Cluster;
- and
- b) diagnosing or monitoring the functional status of the transplant in the patient based upon the expression levels of the genes in the diagnostic gene set.
Dependencies are inferred from the claim text.