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US 2006/0263764 A1Published application
Methods and constructs for evaluation of rnai targets and effector molecules
AssigneeNucleonics, Inc.
InventorCatherine Pachuk
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Abstract
Methods and constructs for selecting double-stranded RNA molecules capable of post-transcriptional gene silencing (PTGS) or RNA interference (RNAi); and methods of selecting targets susceptible to double-stranded RNA mediated PTGS or RNAi.
Claims27
A method for evaluating dsRNA-mediated silencing or inhibition of a target nucleotide sequence by a selected dsRNA effector molecule in an RNAi-competent system, comprising the steps of:
- a) introducing into such system:
- i) a capped and polyadenylated fusion mRNA encoding both a reporter gene sequence capable of translation in said system and a sequence to be evaluated as a target for RNAi (RNAi target sequence) and
- ii) a dsRNA effector molecule having an at least partially double-stranded RNA sequence, one strand of said sequence being substantially homologous to at least a portion of the RNAi target sequence;
- and
- b) detecting the presence of the reporter gene product.
2 more elements
A capped and polyadenylated fusion mRNA encoding both a reporter gene sequence capable of translation in said system and a sequence to be evaluated as a target for RNAi (RNAi target sequence).
Dependencies are inferred from the claim text.