US 2006/0242738 A1 · Polynucleotides and polypeptides in plants

Polynucleotides and polypeptides in plants

AssigneeMendel Biotechnology, Inc.

InventorsBradley Sherman, Jose Riechmann, Oliver Ratcliffe, Cai-Zhong Jiang, Jacqueline Heard, Volker Haake, Robert Creelman, Luc Adam, Lynne Reuber, James Keddie, Omaira Pineda, Peter Repetti, Karen Century, Neal Gutterson, Guo-Liang Yu, Pierre Broun, Roderick Kumimoto, Marsha Pilgrim

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Abstract

The invention relates to plant transcription factor polypeptides, polynucleotides that encode them, homologs from a variety of plant species, and methods of using the polynucleotides and polypeptides to produce transgenic plants having advantageous properties compared to a reference plant. Sequence information related to these polynucleotides and polypeptides can also be used in bioinformatic search methods is also disclosed.

Claims41

Dependent claims

A transgenic plant having increased abiotic stress tolerance as compared to non-transgenic plants of the same species;

wherein the transgenic plant comprises in its genome a transgene encoding a polypeptide member of the G482 subclade of the non-LEC1-like clade of proteins of the L1L-related CCAAT transcription factor family, wherein overexpression of the polypeptide member confers said abiotic stress tolerance.

A method for producing a transgenic plant having increased tolerance to abiotic stress, the method steps comprising:

(a) providing an expression vector comprising
(i) a polynucleotide sequence comprising a nucleotide sequence encoding a polypeptide that comprises a member of G482 subclade of the non-LEC1-like clade of proteins of the L1L-related CCAAT transcription factor family;
wherein
said polypeptide comprises a B domain, and the B domain is sufficiently homologous to the B domain of SEQ ID NO:
88 that the B domain binds to DNA at a transcription regulating region comprising the motif CCAAT;
and wherein said binding regulates transcription of the DNA, and said regulation of transcription confers increased abiotic stress tolerance in the transgenic plant as compared to non-transgenic plants of the same species;
and
(ii) regulatory elements operably linked to the nucleotide sequence, said regulatory elements being effective to control expression of said nucleotide sequence in a target plant;
(b) introducing the expression vector into a plant cell;
(c) growing the plant cell into a plant and allowing the plant to overexpress the polypeptide;
and;
(d) identifying one or more abiotic stress tolerant plants so produced by comparing said one or more abiotic stress tolerant plants with one or more non-transgenic plants of the same species.

9 more elements

A method for producing a transgenic plant having increased tolerance to abiotic stress, the method steps comprising:

(a) providing an expression vector comprising
(i) a polynucleotide sequence comprising a nucleotide sequence encoding a polypeptide that comprises a polypeptide member of the MYB-related transcription factor family;
wherein
said polypeptide comprises a MYB-related domain, and the MYB-related domain is sufficiently homologous to the MYB-related domain of SEQ ID NO:
148 that the MYB-related domain binds to DNA at a transcription regulating region;
and wherein said binding regulates transcription of the DNA, and said regulation of transcription confers increased abiotic stress tolerance in the transgenic plant as compared to non-transgenic plants of the same species;
and
(ii) regulatory elements operably linked to the nucleotide sequence, said regulatory elements being effective to control expression of said nucleotide sequence in a target plant;
(b) introducing the expression vector into a plant cell;
(c) growing the plant cell into a plant and allowing the plant to overexpress the polypeptide;
and;
(d) identifying one or more abiotic stress tolerant plants so produced by comparing said one or more abiotic stress tolerant plants with one or more non-transgenic plants of the same species.

9 more elements

A method for increasing a plant's tolerance to abiotic stress, said method comprising:

(a) providing a vector comprising:
(i) a polynucleotide sequence comprising a nucleotide sequence encoding a polypeptide that comprises a member of G682 subclade of MYB-related transcription factors;
wherein
said polypeptide comprises a MYB-related domain, and the MYB-related domain is sufficiently homologous to the MYB-related domain of SEQ ID NO:
148 that the MYB-related domain binds to DNA at a transcription regulating region;
and wherein said binding regulates transcription of the DNA, and said regulation of transcription confers increased abiotic stress tolerance in the transgenic plant as compared to non-transgenic plants of the same species;
and
(ii) regulatory elements operably linked to the nucleotide sequence, said regulatory elements being effective to control expression of said nucleotide sequence in a target plant;
and
(b) transforming the target plant with the vector to generate a transformed plant with increased tolerance to abiotic stress compared to non-transgenic plants of the same species.

7 more elements

Dependencies are inferred from the claim text.