Modulation of flowering time by the pft1 locus
AssigneeThe Salk Institute for Biological Studies
InventorsJoanne Chory, Pablo Cerdan
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Abstract
The PFT1 (Phytochrome and Flowering Time 1) locus is described and identified. PFT1 acts in a light-quality pathway downstream of phyB that acts through modulation of FT transcription. Plants containing a truncated pft1 gene display an altered shade avoidance syndrome including an increase in time to flowering. The corresponding PFT1 gene has been isolated and characterized. Recombinant vectors, recombinant plants containing the PFT1 gene and methods of using the PFT1 gene to modulate a photosensitive trait, especially time to flowering, are described.
Claims38
A method of modulating at least one photosensitive trait in a plant comprising altering the level of phytochrome and flowering time 1 (PFT1) protein in a plant.
A method of modulating a photosensitive trait in a plant, comprising:
- transforming a plant cell with an expression vector comprising a gene that encodes a PFT1 protein;
- and
- growing said plant cell into a plant under conditions that allow the expression of the PFT1 protein thereby modulating a photosensitive trait.
The method of claim 13, wherein the photosensitive trait is flowering time, and said flowering time is decreased.
A method of modulating a photosensitive trait in a plant comprising:
- contacting a plant cell, or plant, with an inhibitor of a PFT1 gene such that expression of the PFT1 gene is reduced compared to a plant not contacted with the inhibitor.
A transgenic plant having at least one modulated photosensitive trait as compared to a wild-type plant, wherein the transgenic plant comprises a recombinant expression vector that expresses a nucleic acid encoding a PFT1 gene.
A recombinant nucleic acid sequence comprising SEQ ID NO:2.
A recombinant nucleic acid sequence comprising a nucleotide sequence encoding SEQ ID NO:3.
A recombinant nucleic acid sequence hybridizing to SEQ ID NO:2 under stringent wash conditions.
A recombinant nucleic sequence comprising a nucleotide sequence encoding a protein at least 45% to SEQ ID NO:3.
An isolated protein comprising SEQ ID NO:3.
A recombinant nucleic acid molecule encoding a PFT1 protein produced from the method comprising:
- providing nuclear material from a plant;
- and
- isolating from said nuclear material a recombinant nucleic acid molecule encoding a PFT1 protein.
Dependencies are inferred from the claim text.