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US 2006/0242736 A1Published application
Dissimilar promoters for gene suppression
OwnerShihshieh Huang, Thomas Malvar +1inventors
InventorsShihshieh Huang, Thomas Malvar, Larry Gilbertson
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Abstract
Methods of gene suppression comprise transforming eukaryotic cells with recombinant DNA constructs including promoters with dissimilar expression patterns operably linked to one or more gene suppression elements and, optionally, one or more gene expression elements.
Claims22
Transgenic seed having in its genome a recombinant DNA construct comprising:
- (a) a plant endosperm-specific promoter operably linked to at least one first gene suppression element, and
- (b) a plant embryo-specific promoter in the opposite orientation to said plant endosperm-specific promoter and located 3′ to said at least one first gene suppression element.
Stably transgenic plant cells having in their genome a recombinant DNA construct comprising:
- (a) a first promoter operably linked to at least one first gene suppression element for silencing at least one first target gene, and (b) a second promoter that is in the opposite orientation to said first promoter and is located 3′ to said at least one first gene suppression element,
- wherein said first and said second promoters have dissimilar expression patterns, and
- wherein transcription of said recombinant DNA construct in a plant cell results in silencing of said at least one first target gene.
A recombinant DNA construct for transformation of a plant cell, comprising:
- (a) a first promoter operably linked to at least one first gene suppression element for silencing at least one first target gene, and
- (b) a second promoter that is in the opposite orientation to said first promoter and is located 3′ to said at least one first gene suppression element,
- wherein said first and said second promoters have dissimilar expression patterns, and
- wherein transcription of said recombinant DNA construct in a plant cell results in silencing of said at least one first target gene.
1 more element
Dependencies are inferred from the claim text.